Original Article
Phosphorylation of AktSer473/Ask1Ser83 in Trolox-induced suppression of doxorubicin-induced changes in rat cardiomyocytes
Author(s): Anita K Sharma, Ana R Ludke, Ashim K Bagchi, Abd Al-Rahman Al-Shudiefat, Gauri Akolkar, Davinder S Jassal and Pawan K Singal*
OBjECTIvE: To examine the modulatory effects of the antioxidant Trolox in the interplay of Akt and apoptosis signal-regulating kinase (Ask) 1 in doxorubicin (Dox)-induced oxidative stress (OS).
METHODS: Rat cardiomyocytes were treated with Dox (5 μM) or Trolox (20 μM) for 24 h, or were pretreated with Trolox (20 μM) for 4 h before treatment with Dox (5 μM) for 24 h, and were compared with cardiomyocytes without any treatment. For the Akt inhibition study, cardiomyocytes were pretreated overnight with the phosphoinositol 3-kinase/Akt inhibitor wortmannin (1 μM) before treatment with Dox, Trolox or Trolox + Dox. The gain and loss of Akt gene function with HA-tagged constitutively active Akt1 or dominant-negative mutant Akt1 (T308A; S473A) was also studied in the cardiomyocytes under all treatment conditions. Cells exposed to various treatment conditions were analy.. Read More»
DOI:
10.4172/2368-0512.1000003
