Department of Biophysics, Postgraduate Institute of Medical Education and Research, Chandigarh, India

Publications

• Research Article   
  Phospholipase A2 isoforms in benzo (α) pyrene-induced molecular changes in colon cancer cells: A plausible therapeutic target
  Author(s): Sanjeev Kumar Sharma*, Subodh Kumar Yadav, Ujjawal Sharma, Pramod Avt, Satyat Vat Rana and Krishan Lal Khanduja
  
  INTRODUCTION: Colorectal cancer is the third most common cancer prevalent in men, and the second in women worldwide. The exact cause and pathogenesis of this disease remains unknown. In every cell multiple Phospholipase A2 (PLA2) exists, but their contribution to the cell function has yet not been determined. This study has explored the pathways involved in the differential response of HCT-15 and HT-29 colon cell lines during benzo(α)pyrene [B(α)P]-induced molecular changes. METHODS: B(α)P]-induced molecular changes were evaluated through cell viability using MTT assay, Reactive Oxygen Species (ROS) measurement. The gene expressions of PLA2 isoforms were estimated by RT- PCR. Further, knockdown of PLA2 isoform in transfected cells was done with siRNA. RESULTS: The cell viability decreased whereas ROS production increased significantly after exposu.. Read More»
  DOI: 10.37532/pulhhr.22.6(2).42-50

  • Abstract
  • Full-Text
  • PDF