Original Article
High-performance liquid chromatography for the determination of oxymatrine content in radix Sophorae tonkinensis
Author(s): Changli Shao, Chuling Du, Jingsheng Du, Xi Xia, Yinghong Huang, Yu Cai* and Zhihai Huang*
The purpose of the present study was to establish a high-performance liquid chromatography method for the determination of the oxymatrine content of radix Sophorae tonkinensis and to comparatively analyze the content of oxymatrine in radix Sophorae tonkinensis from different origins. C18 was used as a chromatographic column (250 mm × 4.6 mm, 5 μm), methanol/ 3% phosphoric acid in water (80:20) as the mobile phase, 220 nm as the ultraviolet wavelength detection, 1 mL/min as the flow rate and 30°C as the column temperature. All samples were subjected to organic solvent extraction with ultrasonic treatment before the oxymatrine content from radix Sophorae tonkinensis was measured. Oxymatrine in the range of 30 μg/mL to 150 μg/mL had a good linear relationship (r=0.9998), and the average recovery was 99.22%, suggesting that this method is ac.. Read More»
DOI:
10.4172/2529-797X.1000003
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