Correlation between Biofilm Formation and the presence of extended spectrum β-lactamase bla PER1 gene in multiple drug resistant clinical isolates of Acinetobacter baumannii

Joint Event on 2^nd Annual Congress on MICROBIOLOGY AND MICROBIOLOGISTS & 6^th International Conference on MYCOLOGY AND FUNGAL INFECTIONS

October 07-08, 2019 | Madrid, Spain

Nouran Magdy Mohamed Moustafa

Dar Al Uloom University, KSA

Posters & Accepted Abstracts: J Clin Microbiol Infect Dis

Abstract :

Statement of the Problem: Acinetobacter baumannii (A. baumannii) is responsible for most hospital acquired infections. Among the most important A. baumannii virulence factors, is its ability to produce biofilm, which relates to its high degree of antibiotic resistance and survival in hospital environment. The purpose of this study is to identify the ability of different A. baumannii isolates to produce biofilm, and whether this correlates to multidrug resistance and the presence of blaPER-1gene.

Methodology & Theoretical Orientation: A total of 30 isolates of A. baumannii were subjected to susceptibility testing by disc diffusion method for 10 clinically relevant antibiotics followed by phenotypic detection of ESBL production by double disc synergy test (DDST). MIC for imipenem was performed by E-test. Screening for biofilm formation was done by microtitre plate assay. The presence of blaPER-1 was investigated by PCR.

Finding: A. baumannii isolates showed high rate of resistance to the tested antimicrobials. 97.6% were ESBL producers. Sixteen isolates (53.3%) were biofilm producers. There was no significant relation between biofilm formation and MDR. blaPER-1 gene was detected in fifteen isolates (50%) but showed non-significant correlation with biofilm formation and ESBL production.

Conclusion: This study demonstrates the increasing likelihood of A. baumannii isolates to form biofilms. There is no significant association between biofilm formation, MDR and blaPER-1 gene.

Biography :

E-mail: nouran.m@dau.edu.sa