Genomics, transcriptomics, proteomics, and biochemistry of white-rot basidiomycete Trametes hirsuta 072

Annual Congress on Mycology and Fungal Infections

November 16-17, 2017 Atlanta, Georgia, USA

Andrey R Pavlov, Tatiana V Tyazhelova, Konstantin V Moiseenko, Daria V Vasina, Olga V Mosunova, Tatiana V Fedorova, Lilya G Maloshenok, Elena O Landesman, Sergei A Bruskin, Nadezhda V Psurtseva, Alexei I Slesarev, Sergei A Kozyavkin and Olga V Koroleva

A N Bach Institute of Biochemistry Russian Academy of Sciences, Russia Fidelity Systems, Inc., USA N I Vavilov Institute of General Genetics Russian Academy of Sciences, Russia Komarov Botanical Institute, Russia

ScientificTracks Abstracts: Microbiol Biotechnol Rep

Abstract :

Wood-rotting fungi are organisms with the highest natural capacity to degrade lignocellulose substrates, which is enabled by complex systems of extracellular enzymes, whose expression and secretion depend on the nature of the environment. We sequenced and assembled the complete genome of the white rot saprotrophic fungus Trametes hirsuta 072 (Basidiomycota, Polyporales) providing a framework for studies of gene transcription, translation, and secretion of essential fungal proteins. The genome sequence is assembled in 13 chromosomes and a circular mitochondrion, and it is partially annotated. Our transcriptomic and proteomic studies are focused primarily on ligninolytic oxidases and enzymes providing degradation of cellulose and hemicellulose. Our results identified seven laccase genes containing exons and introns, including the respective promoter regions. We found 18 ligninolytic peroxidase genes encoding nine putative lignin peroxidases, seven putative short manganese peroxidases, and two putative versatile peroxidases. The expression of the genes at various conditions of the fungal growth was studied on the transcriptomic and proteomic levels. Only a few genes encoding ligninolytic, cellulolytic, and hemicellulolitic enzymes were expressed in large quantities providing specific response of the fungus to the particular conditions of growth.

Biography :

Andrey R Pavlov is currently working as a Research Scientist in Fidelity Systems, Inc., Gaithersburg, Maryland, USA.