Study of mTOR gene expression in plasma based microRNA-7 / Chitosan Complexes in Hepatocellular carcinoma cell lines

Joint Event on 2^nd World Congress on BREAST CANCER & CANCER SCIENCE AND THERAPY

September 16-17, 2019 | Edinburgh, Scotland

Melek Evirgen

Department of Pharmaceutical Biotechnology, Turkey

Posters & Accepted Abstracts: J Can Res Metastasis

Abstract :

MikroRNAs are small, endogenous and non-coding RNA molecules which regulate gene expression. Recent studies show that deregulation of miRNAs have been associated with different diseases including cancer. Therefore, miRNAs are important therapeutic target in cancer. However, major obstacles of usage miRNAs in therapy are stability, rapid clearance and internalization. For this reason, it is important in terms of therapy with appropriate carrier system to the cells. Aim of this study is to investigate efficacy (invasion, apoptosis and cell proliferation) and usability in hepatocellular carcinoma (HCC) cancer cell lines (Hep3B and HepG2) of polyplexes forms of chitosan and miR-7 mimic. miR- 7 mimic and chitosan complex was prepared. In vitro characterisation of these complex was done. With these dosage have used to investigate mTOR protein levels by using ELISA assays and invasion, apoptosis and cell proliferation assays have done. Our studies show that chitosan/miR-7 complex was internalized stably to cancer cells, thus deregulated miRNA levels repaired. Invasiveness of cancer cells was reduced. Chitosan complexes were shown to be safe and efficient delivery system for miRNA.

Biography :

E-mail: melekevirgen@gmail.com