Barley stripe mosaic virus.
Department of Plant Genetics and Breeding, Islamic Azad University, Iran, Email: Mahdi.tajalifar@yahoo.com
Received: 22-Dec-2021, Manuscript No. PULJPBAS-22-4046; Editor assigned: 25-Dec-2021, Pre QC No. PULJPBAS-22-4046(PQ); Accepted Date: Jan 15, 2022; Reviewed: 29-Dec-2021 QC No. PULJPBAS-22-4046(Q); Revised: 10-Jan-2022, Manuscript No. PULJPBAS-22-4046(R); Published: 24-Jan-2022, DOI: 10.37532/puljpbas.22.4.1.9
Citation: Tajalifar M. Barley stripe mosaic virus. J Plant Biol Agri Sci. 2022;4(1): 1.
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Abstract
The aim of this study was to express TaSTP13 in wheat leaf by (pst). Some abnormal behaviors of TaSTP13 are concentrated in the plasma membrane and act as a homoligomer. Seizure of TaSTP13 reduces wheat susceptibility to Pst by gene silencing (VIGS) by barley bar mosaic virus. Molecular mechanisms and regulatory genes are needed to improve tolerance to environmental stress in products. The new 4-component version (with very high expression) and the 3-component version (with low expression) contain Barley Stripe Mosaic Virus (BSMV) virus-based system which is used for the functional characteri- -zation of finger protein on the C2H2 type in wheat. The four component version, which contains a system based on the barley bar mosaic virus, has a high load capacity for the rapid and stable expression of recombinant protein in various plant species. Excessive expression of TaSTP13 increases a susce- -ptibility of Arabidopsis to powdery mildew and leads to increased glucose accumulation in the leaves.
Keywords
Homo ligomer; Gene silencing; Zinc finger protein; Arabidopsis
Introduction
This study aimed to express TaSTP13 in wheat leaf by Puccinia striiformis f.sp.tritici (pst) For example, is TaSTP13 involved in wheat susceptibility to rust and mildew? Is the decrease in glucose uptake related to wheat resistance due to Lr67? In this study, the expression of TaSTP13 homologs was specifically induced in wheat leaves challenged by the Pst pathotype, CYR31, and abiotic treatments. Intracellular localization analysis shows that TaSTP13 is located in the plasma membrane. Some of the abiotic behaviors of TaSTP13 are concentrated in the plasma membrane and act as hemoligomers [1]. To increase crop yield, genetic and molecular mechanisms that have mechanisms to withstand various environmental stresses should be increased in products. Most genomic research has focused on plant models or products with diploid genomes (such as Arabidopsis and Oryza sativa) [2]. To improve crop performance, special attention should be paid to the underlying genetic and molecular mechanisms of responses and mechanisms for tolerating various abiotic stresses in products. ilencing in transgenic products. This process (gene function) in cereals is a time-consuming method. Plant viral expression systems can be used to rapidly express proteins [3]. BSMV virus is a triple-positive (alfa, beta, gamma) stranded RNA virus with RNAs coated at the end of ‘5 [4]. A Barley Stripe Mosaic Virus (BSMV) virus-based gRNA delivery system targets mutant, wheat, and corn mutants for short, regular, clustered Cross Repetitive Palindromic Replication (CRISPR)/cas9 [5]. The gRNA maintains the Cas9 interaction structure and the ability to identify target genes. In plants, gRNAs direct the Cas9 protein to cut doublestranded target DNA in cells to create double-stranded fractures that can be amplified by a non-identical end-binding pathway and/or a matched repair pathway for repair or mutation. Gives target genes [5].
Materials and Methods
The three- or four-component barley mosaic virus system is used to produce wheat. Functional properties of a finger transcription factor on C2H2 type in response to wheat environmental stress using the new 4-component BSMV system (with very high expression) and the three-component BSMV system is focused on gene regulation. To enhance the expression profile of TaSTP13, a qRT-PCR measurement system is used
Conclusion
The TaSTP13 gene is a protein encoder that carries sugar for wheat. Some of the abiotic behaviors of TaSTP13 are concentrated in the plasma membrane and act as hemoligomers.
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