Honey isolates

Received: 17-Aug-2017 Accepted Date: Aug 19, 2017; Published: 19-Aug-2017

Citation: Esawy MA. Honey isolates. J Microbiol Biotechnol Rep. 2017;1(1):4.

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Editorial

My current research interests since 2011 is focused in honey isolates (bacteria and fungi), my first aim with my team is their identification using phylogenetic systematics, morphological study and other tools. Also, I try to understand their fundamental aspects of their bioactivity. These fundamental aspects include some enzymatic studies since I try to make correlation between the enzymes in honey itself such as amylase, invertase and glucose oxidase and the enzymes produced by honey isolates. My publications [1,2] indicated to a firm relation between the enzyme produced by the honey isolates and bees honey. Also, it was noticed that all the honey isolates bacteria are levansucrase producers [3,4]. Levansucrase is an important enzyme yield levan which play an important role in human health improvement. Our previous study indicated to the importance of levan as antivirus against pathogenic avian influenza HPAI and adenovirus type 40 [3]. Accordingly, examination of levan and their derivatives as strong antioxidant and antitumor agent was done in Esawy et al. [4]. Also, evaluation of honey isolates bacteria as probiotic became my main target in last few years; our preliminary work recommended strongly these types of bacteria to be used as probiotic supplement. Also, my work tries to make a possible relation between the levansucrase, levan and the probiotic bacteria [5].

Enzyme Immobilization

Part of my current research is focused in enzyme immobilization and making comparison study between the free and immobilized enzyme. In the beginning of my work in 1998 [6], I tried to evaluate different immobilization methods and understand the advantageous and disadvantageous of each method. Also, I try to find economic, available and save carriers using different immobilization methods. In 2005 Immobilization of Bacillus licheniformis 5A1 milk-clotting enzyme on on Amberlite IR-120 by ionic binding and I invent a method to recover the carrier and reuse it without facing the clotting problem [7]. I and my team success in 2013 to entrapped all Aspergillus niger NRC1ami pectinase in PVA sponge and the immobilized form showed great ability in orange juice clarification [8]. Also, immobilization of halophilic Aspergillus awamori EM66 exochitinase on Grafted k-carrageenan- alginate was done with great efficiency [9]. Finally, in spite of that dextransucrase is face a big problems in immobilization due dextran yield. I and my team could immobilized Enterococcus faecalis Esawy dextransucrase totally in Fe3+-cross-linked alginate/carboxymethyl cellulose (AC) beads [10].

Enzymic Synthesis

Due to the importance of different molecular weight polysaccharides to be used as prebiotic, in addition to their needing in pharmaceuitical field. I and my team work in enzymic synthesis of levansucrase [11,12]. The study was aimed to control the molecular weights by different parameters and aoolied the most promising polysaccharide in different aspects.

Future Work

Our future work aim to make genetic comparison between honey isolates enzymes and the enzymes from other sources.

REFERENCES

1. Esawy MA, Awad GEA, Ahmed EF, et al. Mansour NM Evaluation of honey as new reservoir for probiotic bacteria advances in food science. Carbohydrate Polymers. 2012;34:72-81.
2. Kansoh AL, Kheiralla ZH, Ahmed HA, et al. Morphological Molecular and Biochemical Comparative Studies of Two Novel Fungal Honey. British Microbiology Research Journal. 2015;5:152-68.
3. Esawy MA, Ahmed EF, Helmy WA, et al. Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans. Carbohydrate Polymers. 2011;36:823-30.
4. Esawy MA, Amer H, Gamal-Eldeen AM, et al. Scaling up characterization of levan and its inhibitory role in carcinogenesis initiation stage Carbohydrate Polymer. 2013;95:578-87.
5. Hamdy AA, Elattal NA, Amin MA, et al. Possible Correlation Between Levansucrase production and Probiotic Activity of Bacillus sp Isolated from Honey and Honey Bee. World J Microbiol Biotechnol. 2017;33:69.
6. Abdel Naby AM, Hashem MA, Esawy MA Immobilisation of Bacillus subtilis œ-amylase and characterization of its enzyme properties. Microbial Res. 1998;153:319-25.
7. Esawy MA and Combet-Blanc Y Immobilization of Bacillus licheniformis 5A1 milk-clotting enzyme and characterization of its enzyme properties. World Journal of Microbiology and Biotechnology. 2006;22: 197-200.
8. Esawy MA, Gamal AA, Kamel Z et al. Evaluation of free and immobilized Aspergillus niger NRC1ami pectinase applicable in industrial processes. Carbohydrate Polymers. 2013;92:1463-9.
9. Esawy MA, Awad GEA, Abdel Wahab WA, et al. Immobilization of halophilic Aspergillus awamori EM66 exochitinase on Grafted k-carrageenan- alginate. Beads 3 Biotech 2016;6:29.
10. Hashem AM, Gamal AA, Hassan ME, et al. Covalent immobilization of Enterococcus faecalis Esawy dextransucrase and dextran synthesis. International Journal of Biological Macromolecules. 82;905-12.
11. Abdel-Fattah AF, Mahmoud DAR and Esawy MA Production of levansucrase f from Bacillus subtilis NRC 33a and Enzymic Synthesis of Levan and Fructo-Oligosaccharides. Current Microbiology. 2005;51:402-07.
12. Esawy MA, Gamal AA, Helal MI, Enzymatic synthesis using immobilized Enterococcus faecalis Esawy dextransucrase and some applied studies. International Journal of Biological Macromolecules 2016;92:56-62.