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Immunohistochemistry [IHC] is an important application of monoclonal as well as polyclonal antibodies to determine the tissue distribution of an antigen [protein or lipid] by specific antigen/antibody reaction tagged with a visible label. Immunohistochemistry has an expanding role in diagnostic and research laboratories. This article highlights the various applications of IHC in health and diseases and gives more information in the Future directions of immunohistochemistry.