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Concerns to the presence of antibiotics in wastewater are pertained to the facts that certain number of antibiotics are resistant to microbial degradation, photolysis, and chemical oxidation. The current works assess the presence of selected antibiotic residues in different soil and plant samples irrigated with tertiary wastewater. Samples of soil and the leafy/edible part of the following local crops were collected from different fields that use tertiary wastewater for irrigation: Raphanus sativus, Raphanus raphanistrum, Allium cepa, Carum petroselinum, Mentha suaveolens and Anethum graveolens. Samples (soil/ root/leaf) were analyzed using the tandem mass spectrometric analysis (LCMS/ MS) technology with limits of detections (LOD) equals to 10 ppb. The study indicated absence of antibiotic residues in tested soil samples and plant tissues.