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The study investigates the expression of epithelial cytokeratin 3 in cultured human keratocytes derived from the limbus and cornea, shedding light on potential mesenchymal-epithelial transition processes. Immunocytochemistry and molecular analysis were employed to assess the presence of cytokeratin 3 in these cells. Our findings suggest the possibility of a mesenchymalepithelial transition in keratocytes, highlighting the intricate cellular dynamics within the ocular surface.