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Metallic nanoparticles are currently widely used in biological and engineering research. Because of their enormous potential in nanotechnology, they have attracted a lot of attention. These materials can now be synthesized and modified with various chemical functional groups, allowing them to be conjugated with antibodies, ligands, drugs of interest, opening up a wide range of potential applications in biotechnology, magnetic separation, preconcentration of target analyses, targeted drug delivery, and vehicles for gene and drug delivery, as well as diagnostic imaging. Furthermore, numerous imaging modalities such as MRI, CT, PET , Ultrasound, SERS and optical imaging have been created over time to aid in the imaging of various disease states. These imaging methods have different techniques and equipment, and they all require a contrast agent with certain physiochemical qualities. For use in these imaging modalities, several nanoparticulated contrast agents such as magnetic nanoparticles (Fe3O4), gold, and silver nanoparticles were developed. In addition, additional multifunctional nano shells and nano cages have been designed to utilize multiple imaging modalities simultaneously.
