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Objective: Hematoxylin and Eosin (H&E) staining and flow cytometry were used to observe the pathological morphology and apoptosis of rat neurons in different functional states to explore the mechanism of electroacupuncture treatment of ischemic stroke.
Method: Thirty male Sprague-Dawley rats were divided into three groups according to the random number table method: normal blank group, model control group, and acupoint group (Baihui, Hegu, Quchi, Zusanli, Sanyinjiao), 10 in each group. The model control group and the acupoint group were treated with focal internal cerebral ischemia model. Rats in the normal blank group and the model control group were fixed without acupuncture during treatment, once a day for 30 minutes, 10 times in a row, and samples were taken after 10 days. The acupoint group was treated with electroacupuncture at 48 hours after operation. The prescriptions were Baihui, Hegu, Quchi, Zusanli and Sanyinjiao. The other acupuncture points except Baihui were connected to the electroacupuncture instrument.
Stimulation for once a day, 30 minutes each time, continuous acupuncture for 10 days, 10 days after the death to take the brain.
Results: Compared with the normal apoptotic rate in the normal blank group, the apoptosis rate of the model control group and the acupoint group increased at an early stage, and the expression of the model control group was higher than that of the acupoint group. The difference was statistically significant (P<0.05); Compared with the normal apoptotic rate in the normal blank group, the apoptosis rate of the model group and the acupoint group increased, and the expression of the acupoint group was lower than that of the model control group, the difference was statistically significant ( P<0.05); Compared with the early and late apoptotic rate of the model control group, the apoptotic rate of the early acupoint group decreased, but the difference was not statistically significant (P>0.05), the late apoptosis rate decreased and the difference Statistical significance (P<0.05).
Conclusion: Electroacupuncture can treat ischemic stroke by inhibiting the apoptosis of nerve cells in the brain.
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