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Volume 2
Journal of Molecular Cancer
Breast Cancer & Vascular Conference 2019
February 25-26, 2019
February 25-26, 2019 London, UK
Joint event on
World Congress on
Breast Cancer
Vascular Biology & Surgeons Meeting
5
th
International Conference on
&
The effect of progesterone administration on the expression of metastasis tumor antigens (MTA1 and
MTA3) in placentas of normal and Dexamethasone-Treated rats
Abrar Al-Atwan
University of Kuwait, Kuwait
A
dministration of Dexamethasone (DEX) induces intrauterine growth restriction (IUGR) in pregnant rats. IUGR can occur
because of apoptosis of trophoblasts, which can be inhibited by progesterone administration. A group of genes called MTAs play
a role in the proliferation and differentiation of trophoblasts. MTA1 upregulates their proliferation and differentiation, while MTA3
downregulates them. Hence, our hypothesis is that during IUGR, MTA1 expression decreases and MTA3 expression increases in
the placenta and this is prevented by administering of progesterone. This study will investigate changes in placental protein content
of MTA1 and MTA3 on 19 and 21 dg in the basal (BZ) and labyrinth (LZ) zones of normal, DEX-treated, and progesterone-treated
placentas. Pregnant rats will be divided into 4 groups: control (V), DEX-treated (D), DEX- and progesterone-treated (DP) and
progesterone-treated (P) groups. All groups will receive daily intraperitoneal injections starting from 15 dg. Animal dissection will be
performed on 19 and 21 dg. Gene expression and protein content of both MTA1 and MTA3 will be studied in the BZ and LZ using
Real-time PCR and Western blotting, respectively.
Abraral36wan@hotmail.comAbrar Al-Atwan, J Molecular Cancer
Volume 2