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Current Research: Integrative Medicine
CAM Therapies 2017
September 18-19, 2017 Charlotte, USA
5
th
International Meeting on
Complementary and Alternative Medicine & Therapies
Pharmacokinetics of major bioactive compounds after oral administration of
Majiepingchuan extracts and transdermal administration of Majiepingchuan cataplasm
in beagles
Xueqian Wang, Fafeng Cheng, Changxiang Li, Yuanjun Liu, Wenxiang Zhu, Xin Du, Xiangjun Yin, Chongyang Ma and Qingguo
Wang
Beijing University of Chinese Medicine, China
M
ajiePingchuan (MJPC) cataplasm, composed of ephedra, mustard, corydalis, bitter almond, and ginger
juice, is a new type of topical preparation in traditional Chinese medicine. Our previous study showed that
MJPC cataplasm extractive could inhibit the degranulation of RBL-2H3 cells activated by compound C48/80 and
treat asthma by enhancing the reactivity of Th1 cells and Tregs, limiting the reactivity of Th2 and Th17 cells, and
regulating the balance of Th1/Th2 cells and Th17 cells/Tregs. To explore the differences between two types of
administration, thepharmacokineticsofmajor bioactivecompounds after theoral administrationofMajiepingchuan
extracts and transdermal administration of Majiepingchuan cataplasm were investigated in beagles. Established
HPLC–MS/MS methods were applied for the pharmacokinetic study of Majiepingchuan cataplasm. The results
showed that ephedrine and pseudoephedrine were able to cross the skin into the bloodstream, while amygdalin,
tetrahydropalmatine, and sinapine thiocyanate could not. In addition, double peaks were observed for the first
time in serum concentration-time profiles of ephedrine and pseudoephedrine after transdermal administration
of Majiepingchuan cataplasm. The mean retention time of ephedrine and pseudoephedrine after transdermal
administration were prolonged compared to that of oral delivery. Bioavailability studies showed that the area
under the curve (AUClast) of ephedrine by Majiepingchuan cataplasm was 1.01 times higher than that by
Majiepingchuan extracts, which indicated that Majiepingchuan cataplasm had higher bioavailability
and long-term efficacy. In this study, we hypothesized the reasons for the failure of compounds to enter
the bloodstream as well as the mechanism underlying the double-peak phenomenon of ephedrine and
pseudoephedrine epimers following transdermal administration of Majiepingchuan cataplasm.
wangqg8558@163.com