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Page 47
Volume 3
August 5-6, 2019 | Singapore
CANCER RESEARCH AND PHARMACOLOGY
STRUCTURAL BIOCHEMISTRY, STEM CELLS AND MOLECULAR BIOLOGY
24
th
International Conference on
International Congress on
&
Cancer Research 2019 & Structural Biochemistry 2019
August 5-6, 2019
Journal of Cancer and Metastasis Research
Chitinase production from
Paenibacillus sp
. BISR-047 utilizing seafood waste as
substrate under solid-state fermentation
Saavi Pradhan
Banasthali University, India
C
hitinases have huge potential applications and biological value in industries; they are used in generating single cell proteins,
sweeteners, insecticides, antifungal drugs, anti cancer agents, biopesticides, food processing agents, degrading agents for
sea waste etc. Chitinases are used for the conversion of chitin a polysaccharide into monomers. Extraction of chitin involves
two steps, demineralisation and deproteinisation, which can be conducted by two methods, chemical or biological. The chemical
method requires the use of acids and bases, while the biological method involves microorganisms.
Solid-state fermentation (SSF) is a low-cost fermentation technology, particularly suitable for the needs of developing countries.
This bioconversion technology of chitinous materials through chitinolytic process is an alternative waste treatment that not only
solves environmental problems but also decreases the production costs of microbial chitinases. Therefore, efforts were made in
the present study to utilize seafood waste for chitinase production under SSF. A novel thermo-tolerant bacterium Paenibacillus
sp. BISR-047, previously isolated from the Great Indian Desert soils, was used and various process parameters were studied.
We obtained 346 IU/ml of chitinase production in a medium containing crab and prawn waste (5:2; waste: water), 1.5 g/kg
yeast extract (w/w), 0.5 g/kg NaCl (w/w), 40% moisture content, pH 8 and at 45 ºC temperature. We obtained 29% dry weight
reduction after 10 d of incubation under SSF. Our results indicate scope for the utilization of seafood waste for industrial
production of chitinase using SSF.
Biography
Saavi Pradhan has her expertise in the field of Microbiology and analytical research studies with experience contribution in
microbiological studies of Great Indian Desert soils. Phytopathogens which cause severe damage to commercial crops were also
studied and successful field trials have been done. Her work also involved pest control biological methodologies to overcome disease
causing pest issues.
sjorwal88@gmail.com