euro-dentistry-2017-keynote

6 / 12

Dentistry: Case Report Euro Dentistry 2017
September 20-21, 2017
Page 20
Notes:
25th Euro Dentistry Congress
September 20-21, 2017 Dublin, Ireland
Margit Schulze, Dentistry: Case Report
Hybrid materials consisting of hydroxyapatite and bio-based polymers used as scaffolds for bone tissue
engineering
Large bone defects require fabricated bone constructs that consist of three main components: an artificial extracellular matrix
scaffold, stem cells with the potential to differentiate into osteoblasts, and bioactive substances, such as osteo-inductive
growth factors to direct the growth and differentiation of cells toward osteogenic lineage within the scaffold. Scaffolds provide a
3D environment for cell seeding and proliferation as well as filling bone defects while affording mechanical competence during
the process of bone regeneration. Today, scaffold development is focused on inorganic-organic composites (hybrids), mainly
prepared using natural and synthetic polymers (i.e. collagen, polysaccharides), and inorganic hydroxyl-apatite (HA), tricalcium
phosphate (TCP). In recent years, tissue engineers used various modifications such as addition of bioactive molecules or
nanoparticles to enhance attachment and proliferation of stem cells on the scaffold. Thus, the application of so called “smart
scaffolds” enhances osteogenic differentiation of stem cells. In Purinergic receptors, P2X and P2Y play a key role in osteogenic
lineage commitment of human mesenchymal stem cells (MSCs) via addition of corresponding P2X/Y receptor ligands
(agonists, antagonists) the differentiation process can be triggered towards osteoblast formation. The focus of this contribution
is the correlations between scaffold structures, both bulk and surface and corresponding cell behaviour, i.e. adhesion and
differentiation. The human MSCs were gained through isolation of jar bone chip and liposuction material harvested during
surgeryintervention. Scaffold structure analysis to investigate scaffold hybrid materials (human, bovine, artificial) provides
information on their chemical composition, 3D bulk and surface structure. Thus, FTIR spectroscopy, X-ray diffraction (XRD),
small angle X-ray scattering (SAXS), scanning electron microscopy (SEM) and zeta potential measurements will be discussed
to explain the hybrid structure-property relationships. Three scaffold materials (collagen, bovine, artificial) were analysed
regarding their chemical composition, 3D bulk and surface structure. Administration of selective P2Y1 antagonists led to an
enhanced matrix mineralization thus confirming the functional role of P2X7 during osteogenesis.
Biography
Margit Schulze has received her PhD at Institute for Organic Chemistry from TH Merseburg/Martin Luther-Universität Halle-Wittenberg in 1990. She hold various position
in her career as a Researcher at Martin Luther University Halle-Wittenberg in 1986, Project Leader at Max-Planck-Institute for Polymer Research Mainz in 1994, Senior
Lecturer at Royal Institute of Technology (KTH) Stockholm in 1996, Head of Industrial Oils, Degussa/Evonik, Darmstadt during 1998-2000 and since 2001 she holds Pro-
fessorship (C3) for Organic Chemistry and Polymers at Bonn-Rhein-Sieg University. She has received Research Award of the Hochschule Bonn-Rhein-Sieg together with
Edda Tobiasch for “Optimaix Bone Regeneration.
Margit Schulze
Bonn-Rhein-Sieg University of Applied Sciences, Germany