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Microbiol Biotechnol Rep | Volume 1, Issue 2
November 16-17, 2017 Atlanta, Georgia, USA
Annual Congress on
Mycology and Fungal Infections
Genomics, transcriptomics, proteomics, and biochemistry of white-rot basidiomycete
Trametes hirsuta 072
Andrey R Pavlov
1
, Tatiana V Tyazhelova
1
, Konstantin VMoiseenko
1
, Daria VVasina
1
, Olga VMosunova
1
, Tatiana V Fedorova
1
, Lilya
G Maloshenok
1
, Elena O Landesman
1
, Sergei A Bruskin
3
, Nadezhda V Psurtseva
4
, Alexei I Slesarev
2
, Sergei A Kozyavkin
2
and
Olga
V Koroleva
1
1
A N Bach Institute of Biochemistry Russian Academy of Sciences, Russia
2
Fidelity Systems, Inc., USA
3
N I Vavilov Institute of General Genetics Russian Academy of Sciences, Russia
4
Komarov Botanical Institute, Russia
W
ood-rotting fungi are organisms with the highest natural capacity to degrade lignocellulose substrates,
which is enabled by complex systems of extracellular enzymes, whose expression and secretion depend on
the nature of the environment. We sequenced and assembled the complete genome of the white rot saprotrophic
fungus
Trametes hirsuta 072
(Basidiomycota, Polyporales) providing a framework for studies of gene transcription,
translation, and secretion of essential fungal proteins. The genome sequence is assembled in 13 chromosomes
and a circular mitochondrion, and it is partially annotated. Our transcriptomic and proteomic studies are focused
primarily on ligninolytic oxidases and enzymes providing degradation of cellulose and hemicellulose. Our results
identified seven laccase genes containing exons and introns, including the respective promoter regions. We found
18 ligninolytic peroxidase genes encoding nine putative lignin peroxidases, seven putative short manganese
peroxidases, and two putative versatile peroxidases. The expression of the genes at various conditions of the
fungal growth was studied on the transcriptomic and proteomic levels. Only a few genes encoding ligninolytic,
cellulolytic, and hemicellulolitic enzymes were expressed in large quantities providing specific response of the
fungus to the particular conditions of growth.
Biography
Andrey R Pavlov is currently working as a Research Scientist in Fidelity Systems, Inc., Gaithersburg, Maryland, USA.
ppav2002@yahoo.com