fungal-infections-2017-keynote

29 / 60

Page 29
Notes:
Microbiol Biotechnol Rep | Volume 1, Issue 2
November 16-17, 2017 Atlanta, Georgia, USA
Annual Congress on
Mycology and Fungal Infections
A comparative study on the growth of Aspergillus species on formulated culture media
from food crop wastes and sabouraud dextrose agar
Janet Uchechukwu Itelima
University of Jos, Nigeria
In developing countries where conventional media used for the isolation and growth of microorganisms are
very expensive and sometimes inaccessible to researchers, there is a growing interest regarding the utilization
of agro-waste materials and other organic wastes for the formulation media used for cultivating microorganisms.
A comparative study was carried out to test the suitability of formulated culture media from food crop waste
materials (yam, sweet potato and potato peels) and that of a conventional medium for cultivating Aspergillus
species isolated from different foodstuffs. Three formulated media which included Yam Glucose Agar (YPGA),
Sweet Potato Peels Glucose Agar (SPPGA) and Potato Peels Glucose Agar (PPGA) were prepared and used in
comparison with Sabouraud Dextrose Agar (SDA) which is a conventional culture medium. One gram of each of
the foodstuffs was disinfected using 1% Sodium hypochlorite solution (1% NaOCl) for one minute, followed by
three successive rinses in sterile distilled water after which they were coarsely crushed and plated directly unto
sterilized Sabouraud Dextrose Agar (SDA). To inhibit bacteria growth, antibiotic drug (gentamicin) in solution,
was added to the media. The Aspergillus species isolated from foodstuffs which included Aspergillus niger,
Aspergillus flavus Aspergillus tamari and Aspergillus fumigatus were aseptically inoculated in duplicate into the
three different formulated culture media including SDA which served as a control. The cultures were incubated
at room temperature (25ºC) for five days. The diameter of the fungal isolates on both the control medium and
formulated media was measured in mutual perpendicular direction to ascertain the redial growth, starting from
the second day to the fifth day of incubation. The four species of Aspergillus species isolated from different food
stuffs grew profusely on the different formulated media with the exception of YPGA which yielded poor radial
growth of the fugal isolates. Although the percentage radial growth of each of the organism on SPPGA and PPGA
did not differ significantly (p˃0.05) from each other, A. niger and A. fumigatus yielded maximum percentage
radial growth of (100%) each on SPPGA and PPGA, while A. flavus and A. tamari yielded (100%) each only
on SPPGA. Fugal growth on YPGA gave lowest percentage radial growth of 50.7, 50.2, 48.6 and 43.5% for A.
niger, A. fumigatus, A. flavus and A. tamari respectively. All the species of Apergillus yielded 100% radial growth
on the control (SDA). As the formulated media, especially (SPPGA and PPGA) compared favourably with the
conventional medium (SDA) in the terms of the radial growth exhibited by the different species of Apergillus, it
is therefore a clear indication that they could be good alternative culture media for the cultivation of these fungal
isolates.
Biography
Janet Uchechukwu Itelima has her expertise in Applied Microbiology and passion in research related to Applied Microbiology, Biotechnology, and Plant Science,
lecturing, and community services. She has obtained her PhD and she is currently an Associate Professor of Applied Microbiology. She is an Academic Staff
of the Department of Plant Science and Technology, Faculty of Natural Sciences University of Jos, Nigeria. She has published 35 papers both nationally and
internationally. She has also written two books. She is deeply involved in motivating students on how to obtain academic excellence. She has attended workshops
and conferences both nationally and internationally, where she presented papers, chaired sessions and served in advisory committee.