microbiology-fungal-infections-2019-scientifictracks

8 / 8

Page 21
Volume 3 Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2 nd Annual Congress on
6th International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
Minimal inhibitory and mutant prevention concentrations of enrofloxacin for Rabbit
Entero-Pathogenic Escherichia coli (REPEC)
Antonella Schiavone, Capogrosso C, Belloli C, Cocciolo G, Camarda A, Romito D and Circella E
University of Bari, Italy
Statement of the Problem : Minimum Inhibitory Concentration (MIC) defines the drug efficacy against bacteria. A novel
approach to minimize the risk of resistance selection is based on Mutant Prevention Concentration (MPC) which neutralizes
potential mutants. Few data about these topics concerning pathogens of some food-producing species such as rabbit are available.
Moreover, the limited number of registered drugs for rabbit farms increases the risk of a frequent use of available molecules,
such as enrofloxacin. The aim of this study was to test the sensitivity to enrofloxacin of Entero-Pathogenic Escherichia Coli from
rabbit’s dead by colibacillosis and to investigate the genetic bases of their resistance.
Methodology & Theoretical Orientation: MIC and MPC values were measured by methods previously described with minor
modifications. Moreover, the sequences of gyrA (DNA gyrase) and parC (topoisomerase IV) genes were analysed according to
previous studies on colonies before and after MPC evaluation.
Findings: Six sensible strains (33 %) were found while 3 (17 %) and 9 (50%) resulted intermediate sensitive and resistant,
respectively. MIC range was 0.008-64 μg/ml. MIC50 and MIC90 were 1 and 64 μg/ml, respectively. MPC values in sensitive and
intermediate sensitive ranged from 0.5 μg/ml to 4 μg/ml (P<0.001), exceeding the clinical sensitivity breakpoint (0.25 μg/ml).
One additional mutation was found in 7 strains after MPC. The double mutation in girA gene (Ser83Leu+Asp87Asn) was
not associated to relevant changes in MPC/MIC values, while a single aminoacid substitution (girA: Ser83Leu) were found
related to high MPC. The combinations of gyrA (Ser83Leu) + parC (Ser80Iso/Gly78Asp/His77Pro) aminoacid substitutions was
associated to to MPC=4xMIC.
Conclusion & Significance: These results highlight the relevance of a correct use of antibiotics to reduce the risk of development
of drug resistant bacteria. Moreover, a therapeutic dosage revision is suggested to minimize the selection of antibiotic resistance
reducing the reservoir of resistant mutants.
Biography
Antonella Schiavone is graduated in Veterinary Medicine at the University of Bari, where she carries out her activity at the Avian
Pathology Unit of the Department of Veterinary Medicine. Her field of expertise includes infectious diseases of rabbits, poultry and wild
animals, and her interests also point to many biological and medical features of the poultry red mite Dermanyssus gallinae, such as
its vectorial role in transmission of infectious diseases, the population dynamics and its epidemiology, its susceptibility to natural and
synthetic acaricides. She also carries out studies about the problem of antibiotic resistance. Additionally, she is experienced in the field
of web communication, with interest towards animal health and behavior.