stem-cells-2019-keynote

Page 12

November 06-07, 2019 | Tokyo, Japan

Volume 02

Journal of Clinical Genetics and Genomics

STEM CELLS AND REGENERATIVE MEDICINE

PEDIATRICS AND CHILD CARE

International Conference on

World Congress on

J Clin Gen Genomics, Volume 02

Stem Cells 2019 & Pediatrics Congress 2019

November 06-07, 2019

• The positive effect was seen rather in the patients having the leukemic cells invaded by CD8+ lymphocytes which

proportion in the marrow cell population declined as leukemia vanished (3053±1036 vs 937±47 × 10E6 cells/L, p<0.070).

The same was with the proportions of CD8+cells co-expressing PD-1 (1238±476 vs 255±73 × 10E6 cells/L, p<0.060).

• The key observation was associated with the analysis of the clonotype profiles (next generation sequencing) which showed

that: (i) dominant clones identified in the recipients of IB-DLI were different from those seen in the lymphocytes prior to

infusion (ii) the dominant clones rather persisted along the observation time even when the leukemia cells disappeared from

the marrow, (iii) the profile of clonotypes in the marrow and in the blood was very similar in 32 out of 50 immunodominant

clones what shows on the similarity between the immune system potential of the blood and marrow lymphocytes, however,

the marrow lymphocyte had their local environment dependent distinctiveness.

In conclusion the IB-DLI (i) is feasibly, (ii) results with the improvement of the patients survival, (iii) is effective rather in those

they have already responded to the leukemic cells with CD8+ cells but they had to be regenerated (PD-1 positivity of CD8+ cells)

by providing fresh cells to achieve reversal of T-cell exhaustionand, finally, to exert clinically relevant activity.

Biography

Andrzej Lange graduated with a medical degree with distinction from the Medical School in Wroclaw, Poland is a professor in the Institute of

Immunology and Exp Therapy of the Polish Academy of Sciences and a founder and head of the Lower Silesian Center of Cellular Trans-

plantation in Wroclaw. His international experience started in 1973-1974 as a Leverhulme fellow in the Middlesex Hospital Medical School,

London. He has been a visitor and lectured in several European and North American scientific institutions. Known from his activity in the

field of bone marrow transplantation and regenerative medicine. Andrzej Lange was awarded several scientific distinctions and served to a

number of National and European institutions were also active in co-editing journals. He is an author and co-author of 249 scientific papers,

in peer-reviewed journals with a cumulative IF of 270 in the years 1995–2017.