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Volume 02
Clinical Pharmacology and Toxicology Research
Toxicology 2019
November 11-12, 2019
November 11-12, 2019 | London, UK
TOXICOLOGY AND CLINICAL TOXICOLOGY
2
nd
International Conference on
Clin Pharmacol Toxicol Res, Volume 02
The impacts of Environmental Chemicals on telomere length and adverse effects in
A549 Cells
Adonay Kiflay
University of Copenhagen, Denmark
H
uman exposure to environmental chemicals is associated with cardiovascular vascular disease, lung cancer, chronic
respiratory disease and mortality. In this study, we investigated the exposure of one group of chemicals (oxidizing agents)
can lead to adverse effects and change the telomere length as a mechanism of health effects in lung epithelial cells in cell cultures.
We selected four oxidizing agents such as Potassium Bromate (KBrO3), Hydrogen peroxide (H2O2), Sodium dichromate
(Na2Cr2O7) and 4Nitroquinoline 1-oxide (4NQO) that can cause DNA damage. The compounds are suggested to be used as
positive controls for the generation of DNA strand breaks and/or oxidatively damaged DNA. We measured cytotoxicity effects
of cell metabolic activity, cell membrane damage and cell proliferation after 24 hours exposure to all chemicals and oxidative
stress levels (ROS generation and antioxidant defense) were measured in short term and long-term exposures after 3 hours
exposure to all chemicals and the effect of long-term exposure on the telomere length. The results can be summarized as (1)
the short-term exposure for KBrO3 leads to low cytotoxicity and oxidative stress effect; (2) the H2O2 causes no cytotoxicity
effects and moderate oxidative stress effect; (3) the Na2Cr2O7 leads to high cytotoxicity and moderate oxidative stress effect
and (4) the 4Nitroquinoline 1-oxide (4NQO) leads to high cytotoxicity and oxidative stress effects. The long-term exposure for
all the chemicals induces moderate oxidative stress effect in the cells. Long term exposure to all chemicals are associated with
no significant change on the telomere length was observed in A549 cells.
e
:
adonay.eyobk@gmail.com