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Volume 2
DEMENTIA AND DEMENTIA CARE
ADVANCES IN ADDICTION SCIENCE AND MEDICINE
July 24-25, 2019 | Rome, Italy
10
th
International Conference on
2
nd
World Congress on
&
Addiction Science 2019 & Dementia Care 2019
July 24-25, 2019
Journal of Clinical Psychiatry and Neuroscience
J Clin Psychiatr Neurosci, Volume 2
Cerebral venules are major factors in formation of brain microinfarcts at systemic
inflammation: Implications for dementia
Gunel Ayyubova, Nigar Guliyeva, Shahla Huseynova, Leyla Yildirim, Shahana Gurbanova
and
Ilaha Sadigi
Azerbaijan Medical University, Azerbaijan
Statement of the Problem
: Despite the widely accepted facts regarding the role of neuroinflammation as well as vascular
pathology in the pathogenesis and progression of neurodegenerative diseases no special significance was given to the pathology
of different cerebral microvessels. The purpose of this study is to describe the structural alterations in neuro-glio-vascular
elements of brain cortex at early stages of acute inflammation.
Methodology
: Systemic inflammation was induced by intravenous injection of purified lypopolysacharide (LPS) from E. coli
[Serotip 0111:B4] at a dose of 1,0 mg/kg dissolved in saline. Semi thin and ultrathin tissue sections were examined by means of
light and electron microscopy.
Findings
: Investigation of brain capillaries revealed insignificant changes in the integrity of their wall. Despite of edematous
perivascular Astrocytic EndFeet (AEF) the architecture of surrounding brain parenchyma was structurally intact. However, the
destructive changes in the wall of cortical venules and their obturation with blood cells were found as indicatives of early onset
of neuroinflammation. Pathological changes were especially intense in the postcapillary venules draining blood from brain
capillary bed and permeability of which is greater than that of other vessels. In fact, the disruption of intercellular tight junctions,
significant increase in the amount of pinocytotic vesicles in endotheliocytes, the appearance of transendothelial channels and
thickening of perivascular basal membranes were indicators of blood-brain barrier disruption in those vessels. The discontinuous
adluminal plasma membrane of endothelial cells and amorphous material in the lumen of venules were observed. Though AEF
around venules were not swollen the integrity of their membranes was disturbed. Moreover, electron microscopy revealed the
apparent indicators of parenchymal damage around venules: degenerative changes in nerve and glial cells, signs of vasogenic
edema among neuropil elements etc.
Conclusion
: the pathology of cerebral venules should be considered as primary causes for brain microinfarcts with subsequent
neurodegeneration at inflammatory conditions.
Biography
Gunel Ayyubova is a senior lecturer, PhD holder at the department of Histology, Embryology and Cytology of Azerbaijan Medical
University. Her research area as the Neuroscience and topic of research is the Immunofluorescence and Electron microscopy of brain
tissue at inflammatory conditions and neurodegeneration.
gunel.ayubova@gmail.com