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Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
De novo transcriptome assembly for gene identification and molecular marker discovery in
Capsicum
annuum
L. exposed at high-intensity UV-B irradiation
Luis Lightbourn
1
, Luis Amarillas
1
, Talia Martinez
1
and Josefina Leon
2
1
Instituto de Investigacion Lightbourn, Mexico
2
Centro de Investigación en Alimentación y Desarrollo, Mexico
Statement of the Problem:
Exposure to high-intensity UV-B irradiation induces the expression
of many genes normally involved in defense, wounding, or general stress responses. However
detailed processes of the linkage between light UV-B signaling and the up-regulation of gene
expression remain unclear. Therefore, the mechanism by which UV-B stress triggers the
intracellular defense signaling pathway remains poorly understood. Moreover, according to our
knowledge, no studies have analyzed the overall changes in global gene expression in bell pepper
leaves exposed to UV-B. Molecular biological analyses have allowed us to draw a picture of UV
stress responses in plants, and determination of the transcriptome has had a significant impact
on this research field.
Methodology & Theoretical Orientation:
Deep sequencing, transcriptome assembly, and differential expression analysis were
performed to investigate the regulatory mechanisms of
Capsicum annuum
in response to UV-B exposure. A global transcriptome
analysis of the response to high-intensity UV-B irradiation was conducted and target genes regulated by UV-B were identified.
Findings:
We conducted a high-throughput screening analysis. After 1 hour, 273 genes showed significantly different expression
between control and treated plants, among these 111 were up-regulated and 162 were down-regulated; these were involved in several
putative metabolic pathways related to biotic stress. After gene annotation and gene ontology enrichment analysis it was possible to
determine that the UV-B radiation induced the expression of genes with functions in UV protection, including antioxidant enzymes,
G proteins, primary and secondary metabolism and transcription factors.
Conclusion & Significance:
Transcriptome profiling highlights possible signaling pathways and molecules for future research. These
results opened ways of exploring the molecular mechanisms underlying the effects of UV-B irradiation on capsicum and have great
implications for further studies.
Biography
Luis Lightbourn is the President of the Instituto de Investigación Lightbourn, Mexico. He is an expert in plant biotechnology, genomics and cell biology and has
over 30 years of experience in plant biochemistry and molecular biology. Throughout his research career he has focused on how light regulates plant growth and
development. In particular, he has made a major contribution to understanding the molecular responses of plants to ultraviolet radiation. He has a range of expertise
that has attracted invitations to contribute to a wide range of activities, including assessment of research strategy, industry consultation and government advice.
lalr@bioteksa.comLuis Lightbourn et al., J Med Biotechnol 2018, Volume 2