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Volume 2
Journal of Medical Biotechnology
Biotechnology 2018
July 16-17, 2018
World Biotechnology Congress
July 16-17, 2018 Berlin, Germany
Characterization of anaerobic biotransformation of β-hexachlorocyclohexane
Mohammad Numan Ibne Asad, Yaqing Liu and Ivonne Nijenhuis
Helmholtz Centre for Environmental Research-UFZ, Germany
H
exachlorocyclohexane (HCH) is a contaminant of concern worldwide. HCH has four main isomers α, β, δ, and γ-HCH. Since
only γ-HCH (Lindane) has a specific pesticide activity, the purification of Lindane resulted in the production of other waste
residues. β-HCH is the most persistent one, has relatively low water solubility and is considered highly carcinogenic and health
hazardous. A large amount of β-HCH produced as a by-product which was dumped at landfill sites has caused heavy contamination
in soil, groundwater and atmosphere. In this study, we focused on the anaerobic degradation of β-HCH. Thus far, only one anaerobic,
Dehalobacter
sp. containing, a culture was reported in the literature. Contaminated soil was collected from a highly contaminated
site in China and anaerobic microcosms were set up to enrich β-HCH degrading microorganisms. The degradation potential was
evaluated by measuring the concentration of the products benzene and Mono Chlorobenzene (MCB). At the same time, cell growth
was monitored by fluorescent microscopy. Illumina sequencing was done for the first and second generation and bacteria belonging
to the
Firmicutes
, including
Dehalobacter, Gelria
and
Gracilibacter
, were dominant. Additionally, the genomic DNA from an active,
fourth generation, the β-HCH degrading culture was isolated and a 16s-rRNA clone library was prepared for subsequent sequencing
to analyze the overall microbial diversity. Furthermore, compound-specific Carbon Stable Isotope Analysis (CSIA) will be applied to
investigate the transformation pathway.
Biography
Mohammad Numan Ibne Asad has recently completed his MS from JLU Giessen on Biotechnology. Especially his MS expertise focused in applied microbiology,
biotransformation, microbial ecology and bioengineering.
numanibneasad@gmail.comMohammad Numan Ibne Asad et al., J Med Biotechnol 2018, Volume 2