breast-cancer-and-cancer-science-2019-posters-abstracts

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J Can Res Metastasis, Volume 3

September 16-17, 2019 | Edinburgh, Scotland

Volume 3

Breast Cancer 2019 & Cancer Science 2019

September 16-17, 2019

Journal of Cancer & Metastasis Research

BREAST CANCER

CANCER SCIENCE AND THERAPY

World Congress on

Study of mTOR gene expression in plasma based microRNA-7 / Chitosan Complexes

in Hepatocellular carcinoma cell lines

Melek Evirgen

Department of Pharmaceutical Biotechnology, Turkey

MikroRNAs are small, endogenous and non-coding RNA molecules which

regulate gene expression. Recent studies show that deregulation of miRNAs have

been associated with different diseases including cancer. Therefore, miRNAs

are important therapeutic target in cancer. However, major obstacles of usage

miRNAs in therapy are stability, rapid clearance and internalization. For this

reason, it is important in terms of therapy with appropriate carrier system to the

cells. Aim of this study is to investigate efficacy (invasion, apoptosis and cell

proliferation) and usability in hepatocellular carcinoma (HCC) cancer cell lines

(Hep3B and HepG2) of polyplexes forms of chitosan and miR-7 mimic. miR-

7 mimic and chitosan complex was prepared. In vitro characterisation of these

complex was done. With these dosage have used to investigate mTOR protein levels by using ELISA assays and invasion, apoptosis

and cell proliferation assays have done. Our studies show that chitosan/miR-7 complex was internalized stably to cancer cells, thus

deregulated miRNA levels repaired. Invasiveness of cancer cells was reduced. Chitosan complexes were shown to be safe and efficient

delivery system for miRNA.