49 / 60
Page 49
Microbiol Biotechnol Rep | Volume 1, Issue 2
November 16-17, 2017 Atlanta, Georgia, USA
Annual Congress on
Mycology and Fungal Infections
Biodiversity investigation and potential of fungal endophytes of peppermint and their
extract effect on chickpea rot pathogens
Kanika Chowdhary
1
, Nutan Kaushik
2
and
Satyawati Sharma
1
1
Centre for Rural Development and Technology, India
2
TERI Institute, India
I
ndia is the highest producer of
Cicer arietinum
(Chickpea), however the crop is highly susceptible to plant
fungal diseases i.e.
Sclerotinia sclerotiorum
,
Botrytis cinerea, Fusarium oxysporum and Rhizoctonia solani
.
For a sustainable and environment friendly alternative, anti-plant pathogenic efficacy of fungal endophytes were
investigated. Endophytic fungal agglomerate of Indian medicinal plant,
Mentha piperita
was investigated for
biodiversity, bio control potential towards chickpea rot causing phytopathogens and their metabolite profiling. 63
pure fungal isolates were recovered from medicinal plant sampled in different seasons from distinct regions of
India. Endophytic fungi were identified by ITS-rDNA sequence process. PCA divulged seasonal variability with
exclusive presence of
Colletotrichum sp., Diaporthe phaseolorum, Alternaria sp., Hypocrea sp. and Rhizopus
oryzae
in second sampling season. Shannon diversity index (H’) was found to be highest in leaf (1.253) from
Mukteshwar. Menhinick’s index discern that stem tissues from Mukteshwar have maximum species richness
(Dmn=1.75). Best antifungal activity was exhibited by extracts of Acremonium sp. (MPM-2.1) with < 1mg/
ml IC50 value towards phytopathogens. GC-MS chromatography of potent biocontrol fungus Acremonium sp.
(MPHSS-2.1) confirmed presence of antifungal compounds 1-heptacosanol and 1-nonadecane.
kanikatokasiit@gmail.com