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Volume 2 Journal of Microbiology and Biotechnology Reports Microbial Biotechnology 2018
September 17-18, 2018
Page 14
Microbial Biotechnology & Vaccine Design
September 17-18, 2018 Lisbon, Portugal
5th World Congress on
John F Alderete, J Microbio and Biotech Rept 2018, Volume 2
The perfect serodiagnostic target: Trichomonas vaginalis STD as model
A rapid, sensitive and accurate serodiagnostic for the number one, non-viral sexually
transmitted infection caused by Trichomonas vaginalis is needed for screening both women
and men. Such a test will also permit determining the true incidence and prevalence of
this STI. Presently there exists the invasive antigen-detection OSOM™ Trichomonas Rapid
Test (Seskui Diagnostics); a lateral flow, immuno-chromatographic Point-of-Care test that
works only for women. During the course of our investigations of the relation between the
vaginalis and prostate cancer, we obtained sera fromwomen and men highly reactive to the
highly immunogenic trichomonad protein α-actinin protein unique to this protist. IgG to
this protein was not detected among uninfected controls. The availability of sera allowed
us to test the hypothesis that the identification of epitopes to other immunogenic proteins
of T. vaginalis would permit the construction of novel, chimeric recombinant proteins that
would be a perfect target for a serum IgG diagnostic for both women and men. We then
identified the immunogenic metabolic enzymes fructose-1,6-bisphosphate aldolase (A), α-enolase (E) and glyceraldehyde-
3-phosphate dehydrogenase (G). Some epitopes of these enzymes were found to have little or no sequence identity to other
eukaryotes, yeasts and microbial pathogens. We constructed a new version of an earlier chimeric recombinant String-Of-
Epitopes (SOE) protein consisting of 15-mer peptides within which were epitopes of A, E and G. This chimeric protein, now
referred to as AEG::SOE2, was detected by ELISA with highly reactive sera of women and men, but not control, negative serum
lacking antibody to T. vaginalis. This approach lends itself to the creation of highly specific immunogenic targets for both
detection of serum antibody in patients and such targets may also be future subunit vaccine candidates.
Biography
John F Alderete has received his PhD from The University of Kansas in 1978 and did Postdoctoral Research at The University of North Carolina at Chapel Hill. He was at
the University of Texas Health Science Center at San Antonio for 30 years before working at Washington State University. He has published 140 scientific articles and 63
book chapters, invited articles and press releases. His work has been presented at 157 scientific conferences and he has given seminars at 90 colleges and universities
worldwide. He has served in National Institutes of Health Study Sections, Boards of Scientific Counselors and National Advisory Councils. He has been a Member of
several National Academy of Medicine panels.
John F Alderete
Washington State University, USA