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Nutraceuticals 2019
July 15-16, 2019
Volume 2
Journal of Food and Clinical Nutrition
Advanced Nutraceuticals and Functional Foods
July 15-16, 2019 | London, UK
World Congress on
J Food Clin Nutr, Volume 2
Exploring the potential of bioactive peptides from
Lamellidens marginali
s for nutraceutical therapy
Jana Chakrabarti, Madhushrita Das
and
Ankhi Halder
A.P.C Roy Government College, India
University of Calcutta, India
University of Calcutta, India
Statement of the Problem:
World Health Organization attributes hypertension as the leading cause of cardiovascular
mortality. Reports suggest that nearly 26% of the adult populations have hypertension worldwide. Dietary changes and
life-style modifications are essential to plan preventive strategies and promote the health of these populations. Dietary
proteins have long been recognized for their nutritional and functional properties. They are good sources of bioactive
peptides with broad spectrum of pharmacological activities. Currently, bioactive peptides from fish and vegetable proteins
are gaining importance for their anti-hypertensive and anti-inflammatory properties. Molluscs, as a group are regarded as
under-exploited source of health-benefit molecules and have good prospects. The freshwater edible mussel Lamellidens
marginalis is considered as an unconventional cheap protein source.
Objective:
Evaluation of the potential of bioactive peptides from Lamellidens marginalis against hypertension and
oxidative stress.
Methodology:
Proximate analyses for nutritive value were done. Protein hydrolysates were prepared using commercially
available protease - Alcalase2.4L. Degree of hydrolysis (DH %) was calculated. ≤ 3kDa peptides of alcalase hydrolysates
(AlcH120) were prepared by ultra-filtration for further study. Anti-oxidative activity was studied with hydroxyl radical and
DPPH radical scavenging activities.AngiotensinConvertingEnzyme [ACE] inhibitory activitywas performed to determine
anti-hypertensiveactivity.MALDI-TOFwasperformedtodeterminetheaminoacidsequenceoftheultra-filtrateofAlcH120.
Findings:
Mussel meat contains moderate amount of protein and carbohydrate but less amount of fat. Mussel protein
hydrolyzed by Alcalase2.4L at 120-minute gives maximum peptide bond cleavage. Progressive antioxidant activity has
been demonstrated with 10mg/ml protein concentration (AlcH120). A novel ACE inhibitory peptide of anti-hypertensive
nature has been identified.
Conclusion & Significance:
Present investigation has demonstrated that bioactive peptides derived from Lamellidens
marginalis can be used for nutraceutical therapy. However, the observations are preliminary and therefore should be
viewed as a prelude to what future holds.