pediatrics-neonatal-healthcare-2017-scientifictracks

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Journal of Pediatric Health Care and Medicine Volume 1, Issue 1
Page 34
September 11-12, 2017 Los Angeles, CA, USA
14th World Pediatrics &
Neonatal Healthcare Conference
Pediatrics & Neonatal Healthcare 2017
Notes:
Autophagy-regulated AMPAR subunit up-regulation in in vitro oxygen glucose
deprivation/reoxygenation-induced hippocampal injury
Autophagy has been implicated to mediate experimental cerebral ischemia/reperfusion-induced neuronal
death; the underlying molecular mechanisms, though, are poorly understood. In this study, we investigated
the role of autophagy in regulating the expression of AMPAR subunits (GluR1, GluR2, and GluR3) in oxygen
glucose deprivation/reperfusion (OGD/R)-mediated injury of hippocampal neurons. Our results showed that,
OGD/R-induced hippocampal neuron injury was accompanied by accumulation of autophagosomes and
autolysosomes in cytoplasm alongside a dramatic increase in expression of autophagy-related genes, LC3 and
Beclin 1 and increased intracellular Ca 2+ levels. Pre-treatment with autophagy inhibitor 3-methyladenine (3-MA)
significantly reduced this effect. Moreover, the OGD/R-induced up-regulation of mRNA and protein expressions
of GluR1, GluR2 and GluR3 were also effectively reversed in cells pretreated with 3-MA. Our findings indicate
that OGD/R induced the expression of GluRs by activating autophagy in in vitro cultured hippocampal neurons,
which could be effectively reversed by the administration of 3-MA.
Biography
Li-Xiao Xu is working as an Assistant Professor of Clinical Research Institute of Soochow University. She got her Doctorate degree in Biochemistry and Molec-
ular Biology at Sun Yat-Sen University in 2012. Since 2012, she has been working in Children’s Hospital of Soochow University and focused on the research of
brain damage, studies on the mechanisms of autophagy in hypoxia-ischemia-induced brain damage. Currently, she has hosted a National Natural Science Foun-
dation-Youth Foundation (No.81502157), and obtained awards from Jiangsu Provincial Medical Youth Talent (No. QNRC2016758) and applied for Foundational
Research of Medical and Health Care of Suzhou City (No.SYS201646). She also have been published many papers.
Li-Xiao Xu
Children’s Hospital of Soochow University, China