std-hiv-aids-2017-posters-accepted-abstracts

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International Journal of HIV and AIDS research

International Conference on

Sexually Transmitted Diseases, AIDS and Parasitic Infections

Parasitology, Infectious Diseases, STDs and STIs

September 21-22, 2017 San Antonio, TX, USA

Cryptosporidiosis in cattle and sheep in Duhok Governorate, Kurdistan region, Iraq

Adel Talib Mohammed Al- Saeed, Jasim Mohammed Abdo

and

Ruwaed Ghanim Gorgees Al-Simaani

University of Duhok, Iraq

he current study is the first one that is performed to detect the oocysts of

Cryptosporidium

in animals in

Duhok Governorate, Kurdistan region of Iraq by examining 348 fecal samples of cattle and 280 fecal samples

of sheep using modified Ziehl Neelsen method (MZNM). All fecal samples were concentrated using formalin-

ether method before staining. A total of 233 fecal samples were randomly chosen to detect

Cryptosporidium

enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) test and 129 fecal samples

from cattle and 104 fecal samples from sheep were taken. By MZNM, the prevalence of

Cryptosporidium

oocysts

was 26.15% in cattle and 11.07% in sheep. The prevalence rates were 28.99% and 24.29% in diarrheic and non-

diarrheic groups of cattle, respectively. While in sheep the prevalence rates were 23.26% and 5.67% in diarrheic

and non-diarrheic groups, respectively. Out of the 129 fecal samples of cattle of which 31(24.03%), 37 (28.68%)

and 57(44.19%) were positive by MZNM, ELISA and PCR, respectively. A total of 104 fecal samples of sheep

of which 23 (22.12%), 24 (23.08%) and 45 (43.27%) were positive by MZNM, ELISA and PCR, respectively.

The highest prevalence rate by MZNM, ELISA and PCR was among the age group 1-2 months in diarrheic cattle

and sheep, while in non-diarrheic cattle and sheep it was among 3-6 months age group. This study indicated that

infection was companied with diarrhea in both the animal species and it decreased with increasing of age, as well

as the asymptomatic infection was common. In present study, the results of sequences analyzing of PCR products

showed identical to the

Cryptosporidium

sequences in the GenBank. In this study, ELISAwas more sensitive and

specific than MZNM which were 82.5% and 90.91% respectively. Also PCR was more sensitive and specific than

MZNM which were 97.5% and 73.82% respectively.