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International Journal of HIV and AIDS research
International Conference on
&
Sexually Transmitted Diseases, AIDS and Parasitic Infections
Parasitology, Infectious Diseases, STDs and STIs
September 21-22, 2017 San Antonio, TX, USA
Diagnostic efficacy of Tsolp-27 recombinant antigen for the serological diagnosis of
neurocysticercosis in Central America and sub-Saharan Africa
Fernando Salazar-Anton 1, Noemia Nhancupe2 and Johan Lindh3
1National Autonomous University of Nicaragua, Nicaragua
2Eduardo Mondlane University, Mozambique
3Uppsala University, Sweden
Cysticercosis is a disease caused by larval stages of Taenia solium (T. solium), which is considered a public
health problem in many low-income countries in Latin America, Africa, and Asia. This illness is now also
emerging in some high-income nations as a result of travel to or immigration from endemic areas. Accurate
diagnosis of neurocysticercosis (NCC) requires costly neuroimaging techniques or commercial enzyme-linked
immunoeletrotransfer blot (EITB), which are seldom affordable for people in endemic countries. Hence, new
low-cost diagnostic methods offering high sensitivity and specificity are needed. The aim of the present study is
to identify, express and evaluate the antigenicity of Tsolp-27 in human sera from Nicaragua and Mozambique.
Immunogenic Tsolp-27 protein from T. solium cysticerci were identified by two-dimensional electrophoresis
Western blotting using human sera from Nicaragua and Mozambique confirmed to be positive for NCC by
computer tomography. The crude Tsolp-27 antigen was sequenced by liquid chromatography-mass spectrometry.
The gene corresponding to Tsolp-27 was cloned, expressed, purified and evaluated serologically. We evaluated
the recombinant antigen Tsolp-27 in relation to commercial and in-house Enzyme-Linked Immunosorbent
assay (ELISA), Western blot-TsolHSP36 and compared them with the EITB that was regarded as the gold
standard method. The analyzed serum samples were obtained from 265 epileptic patients from Nicaragua and
Mozambique, 31 of them were confirmed to be NCC positive by EITB. The serological analysis of Tsolp-27
recombinant antigen in Nicaragua and Mozambique showed a sensibility and specificity of 86.20 % and of
97.24% respectively. Furthermore, considering the simplicity and low-cost of this test, it might be preferable
as a diagnostic method in poorly equipped laboratories in endemic countries. The recombinant protein is now
available and we expect to be useful in the diagnosis of cysticercosis not only in Central and Sub-Saharan Africa,
but also in other endemic regions in the world.