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Volume 3 Journal of Reproductive Biology and Endocrinology WCGO2019
May 13-14 , 2019
Gynecology and Obstetrics
May 13-14 , 2019 Tokyo, Japan
2nd World Congress on
Role of soluble e-cadherin in intrauterine growth restriction
Tyutyunnik Victor L
National Medical Research Centre, Russia
Introduction: Syncytiotrophoblast is an epithelial layer that covers the entire surface of villous tree and
interacts with maternal blood. Its differentiation from chorionic trophoblasts is providing by cell fusion. Thus,
the cell adhesion mechanisms are of particular interest in intrauterine growth restriction studies (IUGR).
E-cadherin is a cell adhesion molecule with molecular weight of 120-kDa that is important for epithelial
intercellular adhesion. Soluble form (80-kDa) consists of Ca-dependent proteolysis and can reduce cell
adhesion.
Objective: The aim was to study the levels of soluble (80-kDa) and transmembrane (120-kDa) forms of
E-cadherin in placentas of IUGR and non-IUGR fetuses.
Materials and methods: 38 pregnant women, among them 18 affected by Intrauterine Growth Restriction
(IUGR) and 20 control subjects from normal pregnancies. The Western blotting was used for determination
the levels of soluble and transmembrane E-cadherin in placentas. Measurement of E-cadherin gene expression
was made by real-time polymerase chain reaction CFX96 («BioRad», USA). The statistical analysis was
performed with programs AtteStat and OriginPro 8.1.
Results: In IUGR group the level of the transmembrane form of E-cadherin was reduced by 2.5 times and the
soluble form by 4.8 times (p <0.05) compared to normal placentas. Their ratio was of 1.6 in the IUGR group
and 0.8 in control group (in both cases p <0.05). We studied the expression of the E-cadherin gene CDH1.
The level of expression of the E-cadherin gene CDH1 in the placentas from pregnancies with fetal growth
restriction was reduced by 1.4 times compared to the control group (p>0.05). The absence of significant
differences indicates the possibility of post-translational modification of E-cadherin.
Conclusion: Thus, low levels of soluble E-cadherin in placentas are associated with decreased proliferation
and mobility of trophoblast, which may be the cause of placental insufficiency and, consequently, IUGR.
J Reprod Biol Endocrinol, Volume 3