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Current Research: Integrative Medicine
CAM Therapies 2017
September 18-19, 2017 Charlotte, USA
5
th
International Meeting on
Complementary and Alternative Medicine & Therapies
Antihypertensive activity of flowering twigs of
Calotropis procera
(Ait.)
Niaz Ali, Wahib Ullah
and
Salman Khan
Khyber Medical University, Pakistan
Background:
Young flowering twigs of
Calotropis procera
are used in treatment of hypertension in the local
tribes of Kohat regions, Pakistan. The current work is an attempt to document scientific rationale for the traditional
anti-hypertensive use of flowering twigs of
Calotropis procera
.
Methods:
Acute toxicity study of crude methanol extract of
Calotropis procera
was performed using mice model.
The crude methanol extract and its fractions were screened for possible antihypertensive activity in experimentally
induced HTN in rats. Rabbits’ aortic strips were tested in presence and absence of I µM of norepinephrine. The
tests samples were also screened on the KCl (80mM) - induced contractions and a tension of 1 g in intact tissues.
The same series of experiments were carried out in denuded aortic strips to find out mechanisms. Phytochemical
screenings were also performed.
Results:
Phytochemical screenings suggested for the presence of saponins abundantly.
Calotropis procera
produced significant fall in hypertension (P≤0.05). The Aortic stripes were relaxed by the test samples both on
NE induced contractions and KCl induced contractions. However, denuded tissues were not relaxed suggesting
the involvement of NO releasing mechanism. The 1 µM NE induced contractions were relaxed that suggests for
the involvement of receptors operated channels. Relaxing effect on the KCL induced contraction also suggest the
inhibition of voltage gated calcium channels (P≤0.05).
Conclusion:
Flowering twigs of
Calotropis procera
have antihypertensive activity which is mainly due to
saponins.
niazpharmacist@yahoo.com