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Joint Event
November 29-30, 2019 | Frankfurt, Germany
28
th
International Conference on
3
rd
International Conference on
Diabetes and Endocrinology
Diabetes and Metabolism
&
2
0
1
9
CONGRESS
DIABETES
2019
DIABETES
Journal of Endocrine Disorders & Surgery | Volume 3
Investigation of molecular mechanism of glucose-lowering activity of Aquilaria crassna L.
leave extract in HepG2 cells
Phanupol Mongkolsiri
Mahidol University, Thailand
T
ype 2 diabetes mellitus is a chronic metabolic syndrome
characterized by hyperglycemiawhich can further develop
microvascular and macrovascular complications. Aquilaria
crassna L. was reported as a traditional Thai medicinal plant
that possesses an anti-diabetic effect in animal model. The
present study revealed the mechanism by which this plant
extract lowered glucose levels in cultured hepatocellular cell
line, HepG2. ALE at a concentration of 6.25, 12.5 and 25 µg/
mL significantly increased glucose consumption in a dose-
dependent manner. The increase of glucose consumption of
ALE-treated HepG2 cells was accompanied by 2-fold increase
of the glycogen synthase 2 (GYS-2) mRNA expression and 50%
suppressing of glucose-6-phosphatase expression. These data
indicate that the anti-diabetic effect of ALE on HepG2 cells is
mediated through the increased of glycogen synthesis and
the decrease of gluconeogenesis. Gas-chromatography and
mass spectrometry (GC-MS) analysis of the ethanolic extract
of ALE consisted of nitrogen containing compounds (39%),
terpenoids (31%), polyketide (18%), and phenylpropanoids
(12%). Investigation of glucose-lowering effect of these
compounds is currently underway.
Mechanism of glucose-lowering effect of A. crassna leaves extract
(ALE) in HepG2 cells, (↑; Increase, ; Decrease
Speaker Biography
Phanupol Mongkolsiri is a graduate student in the Biochemistry Program
at Mahidol University where he investigates biolochemical activities of
natural products which may be used as anti-diabetic drugs. He completed
his
B.Sc. in Chemistry fromMahasarakham University.
e:
phanupol.mon@student.mahidol.ac.th