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Page 45

Microbiol Biotechnol Rep | Volume 1, Issue 2

November 16-17, 2017 Atlanta, Georgia, USA

Annual Congress on

Mycology and Fungal Infections

Detection and diagnosis of wood decay fungi in wooden heritage using different image

techniques

Alfieri, Paula Vanesa

and

Correa M Veronica

LEMIT, Argentina

T

his study was based on the current deteriorated status of wood slats from locomotive turntable of Provincial

Railway Station La Plata. Wood specie of the slats was determined by conventional methods being

Schinopsis

sp

. while fungal species was determined morphological being

Phellinus chaquensis

(white-rot fungus).

Determination of the fungus and its

in-vitro

cultural features were based on Iaconis and Wright and Robledo

and Urcelay. Fungal degradation wants be measured by non-destructive methods: area occupied by mycelium

and basidiomata were observed by x-ray radiography and computer tomography (CT) and quantified by image

analysis with Image J software. Greyscales of the images obtain indicated density changes, being black scale the

less dense and white scale the densest. To establish the microstructural wood deterioration (cell wall), scanning

electron and optical microscopy (SEM and OM) images were analyzed. It was concluded that deterioration

analysis by images is a non-destructive alternative methodology, which allows to measure structural condition

of material. This is essential in heritage conservation because it allows defining correctly the deteriorated status

useful to planning a conservation strategy, avoiding the asset loss.

paulaalfieri@gmail.com