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Page 43
Microbiol Biotechnol Rep | Volume 1, Issue 2
November 16-17, 2017 Atlanta, Georgia, USA
Annual Congress on
Mycology and Fungal Infections
Diversity of
Aspergillus
species associated with groundnut
(Arachis hypogaea L.
) in
eastern Ethiopia as revealed by InDels and their potential for aflatoxin production
Abdi Mohammed
1
, Alemayehu Chala
2
, Mashilla Dejene
1
, Chemeda Fininsa
1
, Chris Ojiewo
3
, Amare Ayalew
4
, David A Hoisington
5
,
Victor S Sobolev
6
and
Renee S Arias
6
1
Haramaya University, Ethiopia
2
Hawassa University, Ethiopia
3
ICRISAT, Ethiopia
4
African Union Commission, Ethiopia
5
University of Georgia, USA
6
Peanut Research Laboratory, USA
A
spergillus
species cause aflatoxin contamination, which becomes a health threat in agricultural products and
leads to commodity rejection by domestic and international markets. Hence, it is necessary to discriminate
diversity and aflatoxin producing species in the eastern Ethiopia. Therefore, the current study was undertaken
to elucidate the genetic diversity of
Aspergillus
isolates through InDel (Insertion and Deletions of sequences)
markers and evaluate
in vitro
aflatoxin production abilities of the isolates using YES (Yeast Extract Sucrose)
medium. A total of 276 isolates were used for genetic diversity fingerprinting of DNA using 23 InDel markers
based on aflatoxin biosynthesis gene cluster. Cluster analysis was analyzed by NJ (neighbor joining) and by
PCoA (Principal Coordinate Analysis). The
Aspergillus
isolates studied in the current work grouped into three
clusters. In addition, 269 isolates were tested for aflatoxin production using UPLC (Ultra Performance Liquid
Chromatography). Aflatoxigenic isolates had a maximum of 247 µg mL-1 aflatoxin B1 and 139 µg mL
-1
aflatoxin
G
1
. This study provides insight into the genetic biodiversity of aflatoxin biosynthesis gene cluster of
Aspergillus
in relation to
in vitro
aflatoxin production of isolates in the country. The prevalence of aflatoxigenic isolates was
much higher (93% of the tested isolates) than the non-aflatoxigenic.
abdi.mohammed22@yahoo.com