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Volume 2
Journal of Microbiology and Biotechnology Reports
Microbial Biotechnology 2018
September 17-18, 2018
Microbial Biotechnology & Vaccine Design
September 17-18, 2018 Lisbon, Portugal
5
th
World Congress on
Prevalence and control of
Cronobacter sakazakii
containing putative virulence genes
Shruti Sharma and Alka Prakash
Dayalbagh Educational Institute, India
Cronobacter sakazakii
is an emerging food-borne pathogen that is associated with a number of infections in infants, neonates
and immune-compromised individuals. It is associated with a number of life-threatening diseases like necrotizing enterocolitis,
sepsis, meningitis, bacteremia, CSF infection, brain abscess and cyst formation. In the present study prevalence of C. sakazakii was
investigated in dairy products of Agra city, India. Total 243 dairy product samples were analyzed in different seasons and a total of 480
isolates were obtained. These isolates were biochemically characterized and were further confirmed using 16S rRNA gene sequencing.
The isolates were subjected to
in vitro
and
in vivo
pathogenicity testing. The presence of virulence associated genes was checked in
these isolates and the effect of various stresses like acid, alkaline, heat, cold and desiccation was also studied on these isolates. qPCR
based studies were conducted to check the expression of putative virulence gene under stressed and unstressed cells. A number of
antibiotics and probiotics were tested to control these
C. sakazakii
isolates. Amongst antibiotics Ofloxacin, Piperacillin, Cefotaxime
and Chloramphenicol were the most effective antibiotics and amongst probiotics
L. fermentum
and
Pediococcus acidilactici
were the
most effective in controlling the
C. sakazakii
isolates.
shruti6591@gmail.comShruti Sharma et al., J Microbio and Biotech Rept 2018, Volume 2