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Volume 2
Journal of Microbiology and Biotechnology Reports
Microbial Biotechnology 2018
September 17-18, 2018
Microbial Biotechnology & Vaccine Design
September 17-18, 2018 Lisbon, Portugal
5
th
World Congress on
Maria Camila Escobar et al., J Microbio and Biotech Rept 2018, Volume 2
Reduction of selenite to selenium using bacteria isolated from polluted areas
Javed Saima
1
, Faisal Muhammad
1
and Naeem Muhammad Salman
2
1
University of the Punjab, Pakistan
2
National Textile University, Pakistan
In this work toxic selenite which is also highly soluble is transformed to selenium a less toxic element. Selenium is transformed through
biotic transformation using different strains of bacterial like
Pseudomonas, Exiguobacterium sp
.,
Bacillus subtilis
and
licheniformis
.
Selenium can exist in different forms like reduced form (Selenide, Se2-), water dissolved form (Selenite, So3-2/Selenate SeO4-2) and
in the form of element (Seo). Different physical parameters were changed for optimizing conditions like different concentrations of
Selenium (Se) varying from 200 to 400 and finally to 600 µg ml-1, temperature, pH, aeration along with incubation time for high
reduction of selenite. It was found that selenite reduction rate was increased by increasing pH. It was found that at pH 3 around 15-
33% Selenite was reduced and this trend kept on increasing to 28-90% at pH 9. For evaluating optimum temperature for Selenite
reduction three levels of temperature were selected (32 °C, 37 °C and 42 °C) were selected. The selenite reduction was found at
different temperatures and the results showed that for optimum reduction of selenite all strains possess varying preferences. The
reduction in Selenite was also checked at different concentrations of selenite and it was found that maximum reduction of selenite
was observed at lower concentration. This study concluded that in aerobic and anaerobic environment Se can be remediated by using
selenite reducing bacteria.
saima88javed@gmail.com