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Volume 3
Journal of Pharmacology and Medicinal Chemistry
Nanomedicine 2019
Biotechnology 2019
May 20-21, 2019
May 20-21, 2019 London, UK
4
th
World Biotechnology CONGRESS
Nanomedicine and Nanotechnology
4
th
International Conference on
&
Waste Vs Cancer: An innovative biomedical approach
Ram Kumar Ganguly
Vidyasagar University, India
V
ermitechnology involves vermiculture and vermicomposting process which emerged as a comprehensible and lucrative
technology for management of organic wastes. During vermicomposting, waste materials while passing through
the earthworms gut are homogenized in a highly bacterial rich environment into a highly mineralized chemical forms.
The present study reflects compost as an incubator of different beneficial bacteria which opens up a new vista not only in
vermitechnology but also its biomedical significance. A significant turnover of different enzymes such as leucine arylamidase,
β-Glucosidase, Urease and α-celulase supported further by FT-IR analysis represents a changeover in compost. The bacterial
metabolic products allied with earthworms have an exclusive physicochemical pre-eminence as a facilitator of apoptosis
(programmed cell death in a MCF-7 cell line). Five strains of bacteria were isolated and identified using 16srRNA sequencing.
The ethyl acetate extract of one of the bacteria Bacillus anthracis have been undertaken which show a significant anticancer
and antioxidant activity. Antioxidant activity revealed through DPPH radical scavenging assay show a maximum effect of
(75.79 ± 5.41%) at 900 μgml-1 concentration of the extract. Furthermore, a significant decrease in SOD and LDH activity
were noticed upon application of extract. MTT assay showed a potent cytotoxic activity against MCF-7 cell line with an IC50
value of 46.64 ± 0.79 μgml-1. Apoptosis was further confirmed through the nuclear fragmentation and DNA fragmentation
analysis. Western blot analysis represents a down regulation of Akt protein, phosphorylated FOXO proteins. Increase of
SOD activity along with decrease of Akt level reflects that the mode of action is entirely PI-3K dependent. It indicates that
B. anthracis isolated from vermicompost could be potentially explored for the development of new therapeutic agents,
especially against cancer. It will definitely open up a new vista in the area of waste management and bio-medical innovations.
Biography
Ganguly R.K. has completed Master of Science from Banaras Hindu University, India and had qualified several fellowship programs like
CSIR-JRF, ICMR-JRF, GATE-JRF and SET JRF. He is now conducting the research work from Vidyasagar University and published a
couple of international journals in different fields of biotechnology.
ramkumarganguly10@gmail.comJ Pharmacol Med Cheml, Volume 3