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Page 22
Volume 3
Journal of Pharmacology and Medicinal Chemistry
Nanomedicine 2019
Biotechnology 2019
May 20-21, 2019
May 20-21, 2019 London, UK
4
th
World Biotechnology CONGRESS
Nanomedicine and Nanotechnology
4
th
International Conference on
&
Development of galactosamine decorated andrographolide loaded nanocochleates for
liver cancer targeting
Bothiraja Chellampillai
Poona College of Pharmacy, India
Statement of the Problem:
Andrographolide (ANG), an anticancer chemotherapeutic phytoconstituent has been used in the
treatment of various tumours and produced 39% inhibition cancer cell due to lack of specific affinity for site of action or to its
limited biopharmaceutical properties. This juncture demands an effective, controlled release and safe formulation of AND would
be a significant advance for the treatment of cancer. Nanocochleates are unique lipid-based supramolecular assemblies composed
of a negatively charged phospholipid and a divalent cation. Aim of the study was to develop galactosamine (GA) decorated
andrographolide (ANG) loaded nano-cochleates (NC) for liver targeting.
Methodology:
GA was attached to ANG-loaded 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) nanoliposomes (GA-
ANG-NL) by aldehyde chemistry. GA-ANG-NL was converted into nanocochleates (GA-ANG-NC) by addition of Ca2+ ions
and evaluated in terms of in-vitro and in-vivo and compared with ANG and ANG-NC.
Findings:
ANG-NC and GA-ANG-NC showed particle size of 149 and 835 nm and zeta potential of -0.308 and -2.08 mV,
respectively. ANG-NC and GA-ANG-NC showed higher release in pH 5.3 as compared to pH 7.4. GA-ANG-NC demonstrated
higher in-vitro anticancer activity in Human hepatoma cell line Hep-G2. The targeting effect for the GA-ANG-NC was also
demonstrated in which fourfold improved GI50 as compared ANG. Moreover, bioavailability of AGN from GA-ANG-NC
increased by 3-fold with long circulation time and slower plasma elimination. Furthermore, GA-ANG-NC showed 2.1-fold
increases in liver drug concentration ANG.
Conclusion & Significance:
The proposed strategy is advantageous in terms of targeted drug delivery and has high potential
to address the current challenges in drug delivery. Thus, the prepared nanocarrier offers a novel formulation that combines the
unique properties of a biodegradable material, galactosamine and nanocochleates for biomedical applications.
Acknowledgement:
The authors sincerely acknowledge Lipoid GmbH, Ludwigshafen, Germany for providing gift sample of1,2-
dioleoyl-sn-glycero-3-phospho-L-serine (DOPS).
Biography
Bothiraja has expertise in the field of novel and targeted drug delivery systems. His rigorous research work has been dedicated in
various research projects like nanoparticulate systems drug delivery, tumor targeting, solid dispersion and crystal engineering. He has
50 research papers published in various international and national journals depict quality, innovativeness and expertise achieved by
him in mentioned research fields. He would also like to use his enthusiasm for science to involve students and help them to become
successful and contributing members of the scientific community.
pounbothi@yahoo.comJ Pharmacol Med Cheml, Volume 3