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Recycling 2019 & Material Science 2019
July 22-23, 2019
Volume 3
Journal of Environmental Geology
Material Science and Nanotechnology
Global Recycling Summit
July 22-23, 2019 | Rome, Italy
6
th
International Conference on
&
Formulation of non-ionic surfactant vesicles (NISV) prepared by microfluidics for therapeutic delivery
of siRNA into cancer cells
Mohammad Ali Obeid
1
, Alexander B Mullen
2
, Rothwelle J Tate
2
, Valerie A Ferro
2
1
Yarmouk University, Jordan
2
University of Strathclyde, UK
Introduction:
RNA interference involves the degradation of a target messenger RNA through the incorporation of short
interfering RNAs (siRNA) [1]. The application of siRNA based therapeutics is limited by the development of an effective
delivery system. A novel type of nanoparticles known as non-ionic surfactant vesicles (NISV) are commonly used for drug
delivery of various therapeutics, are relatively safe and non-expensive, have not been extensively studied for siRNA delivery [2].
Therefore, the aim of this study was to investigate the potential of NISV prepared by microfluidics for siRNA delivery.
Methods:
NISV were prepared by microfluidic mixing which is a recently developed method used to prepare lipid based
nanoparticles and results in the production of small vesicles with efficient encapsulation of a therapeutic agent. To prepare
NISV, specific volumes from each stock solution of the NISV components were mixed together to prepare the lipid phase. The
lipid phase was injected into the first inlet and the aqueous phase into the second inlet of the microfluidic micromixer, with
the mixing temperature set at 50°C. The flow rate ratios (FRR) between the aqueous and organic phase was set at 3:1 and the
total flow rates (TFR) of both phases was set at 12 ml/min. This allows for fast mixing between the two phases at high flow
rates and at a temperature above the phase transition of the lipids. Dispersions were then collected from the outlet stream and
immediately diluted in order to reduce the final ethanol content in the preparation to 6.25% (v/v). Cytotoxicity evaluation of
NISVwere carried out on non-small lung cancer cells (A549) and mouse melanoma cells (B16-F10-LUC). siRNA targeting green
fluorescent protein (GFP) in copGFP-A549 cells, or luciferase in B16-F10-LUC cells were encapsulated in NISV. Inhibition of
GFP expression by anti-GFP siRNA (siGFP) delivered using NISV was evaluated by flow cytometry, polymerase chain reaction,
and Western blotting. Nude BALB/c mice inoculated with B16-F10-LUC cells that induce melanoma expressing luciferase was
used to assess the NISV ability to deliver siRNA in vivo.
Results:
Cytotoxicity studies indicated that NISV were not toxic at or below 40 µg/ml. NISV formulations had high siRNA
encapsulation efficiency. Fluorescent microscope and flow cytometry studies indicated high cellular uptake by the cells
compared to naked siRNA, which was not taken up by the cells. NISV were able to deliver siGFP to the cells and significantly
suppress GFP expression. These results were confirmed by transfecting the luciferase producing B16-F10-LUC cells with anti-
luciferase siRNA (siLUC). Measuring the level of luciferase expression after siLUC transfections using a luciferase protein assay
system successfully demonstrated the suppression of luciferase expression. NISV were then used in in vivo experiments using
nude BALB/c mice. After intra-tumoural injection, siLUC was delivered to the cells and suppressed luciferase expression at a
significantly higher level than mice treated with naked siLUC. These in vivo results confirm the ability of NISV to successfully
deliver siRNA into the cytoplasm of the target cells and suppress the target protein.
Conclusion:
NISV have been demonstrated extensively and for the first time to have the potential to be used as a delivery system
for siRNA. These results have shown that NISV can be used to overcome the barriers, such as low stability and poor cellular
uptake, in siRNA-based therapeutics.
JEnvironGeol. |Volume3
ISSN:2591-7641