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Volume 02

Journal of Immune Disorders & Therapy

J Immune Disord Ther, Volume 02

December 09-10, 2019 | Barcelona, Spain

9

th

WORLD CONGRESS ON

IMMUNOLOGY AND CANCER

World Immunology 2019 & Cancer Summit 2019

December 09-10, 2019

Angiotensin II induces Reactive Oxygen Species (ROS) generation and apoptosis in

lymphoma EL4 cells

Maha Aljumaa

King Abdullah International Medical Research Centre, KSA

Background/aim

: Renin angiotensin system (RAS) is implicated in cardiovascular diseases.Ang II infusion induces hypertension

and vascular damage especially endothelial dysfunction, inflammation and oxidative stress. Angiotensin II receptors are

expressed in immune cells like T cells and monocytes. Tumour become more aggressive and dangerous in parallel to angiogenesis

development witch induce intravasation and extravasation cells going from primary tumour to blood and then from blood to

colonize new site and induce metastasis. Macrophages play a pivotal role in inflammation and cancer microenvironment. We

aim to study the effect of angiotensin II in Macrophage polarization and cancer microenvironment related to innate immunity.

Materials and methods

: Malignant lymphoma EL4 cells were incubated for 24h, 48h and 72h with conditioned media of

macrophages stimulated with Angiotensin II (1Um) for 24h. We determine the effect of Angiotensin II stimulate macrophages

in Reactive Oxygen Species (ROS) generation using CellRox kit, viability and apoptosis using immunofluorescence and cell

phenotyping using flow cytometry.

Results and conclusion

: There was a significant increase in ROS generation in cells incubated with Angiotensin II compared

to control. Angiotensin II increases significantly apoptosis time dependently from 24 to 72h and change cell CD inflammatory

markers after 24h incubation. These differences may be caused by significant increase in ROS generation in cells incubated with

Angiotensin II compared to control.

mahaaljumaa@hotmail.com