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Microbiol Biotechnol Rep | Volume 1, Issue 2
November 16-17, 2017 Atlanta, Georgia, USA
Annual Congress on
Mycology and Fungal Infections
Draft genome sequence of 11 clinical and environmental Colombian isolates of genus
Aspergillus
Gomez OM
1,2
, Freyle C G
2,3
, Tamayo D P
2,3
, Rua AL
2,3
, McEwen J G
2,4
and
Hernandez O
2,3
1
Universidad de Antioquia, Colombia
2
Corporación Para Investigaciones Biológicas, Colombia
3
Universidad de Antioquia, Colombia
4
Universidad de Antioquia, Colombia
Background:
The genus
Aspergillus
is a group of opportunistic fungi that cause infections, with high morbi-
mortality in immunosuppressed patients. Approximately 350 species have been described in this genus, classified
in 4 subgenus and 20 sections. In Colombia,
Aspergillus
fumigatus
is the most frequent species in these infections.
However, in the last years an increase in the incidence of other species has been observed. This added to an
increase in the quantity and diversity of
Aspergillus
isolates in hospital environments.
Aims:
Characterize the phenotype and genotype of clinical and environmental Colombian isolates of
Aspergillus
spp.
Methods:
We collected 11 Colombian isolates of
Aspergillus
: 4 from diferents clinical samples, six from hospital
environments and 1 from extra-hospital environments. The isolates were identified according to their macro and
microscopic characteristics. Genomic DNA for sequencing was prepared from mycelium culture using phenol/
chloroform extraction. Library preparation and 150-bp paired-end sequencing was performed using the Illumina
HiSeq 2500 platform. The reads was de novo assembled using SPAdes 3.10 pipeline. The draft genome assembly
quality was analyzed by QUAST. Augustus v3.0.1 and GlimmerHMM were used for gene prediction. Inference
by sequence homology was performed with OrthoFinder v2.0.9. IQtreev1.4.4 software was used for phylogenetic
reconstruction by ML.
Results:
In this study, four of the eleven isolates classified phenotypically were re-classified correctly after
genome analysis. We obtained whole genome sequence of 4 clinical and 7 environmental isolates, including 3
novel genome sequences of the genus
Aspergillus
not reported in the databases:
A. tritici (Clinical), A. tamari
(hospital environment) and
A. amoenus
(extra-hospitalenvironment).
Conclusion:
The results of this study provide useful data for the genomic comparison between clinical and
environmental isolates of
Aspergillus
. Valuable information that can be used to understand the evolutionary
relationships, determine the diversity and level of production of enzymes and secondary metabolites, predict the
pathogenicity and response of this opportunistic fungus to antifungal agents.
Biography
Gomez OM is currently working as Faculty at Universidad de Antioquia, Colombia.
oscargomez24@hotmail.com