Previous Page  11 / 12 Next Page
Information
Show Menu
Previous Page 11 / 12 Next Page
Page Background

Page 34

Notes:

Microbiol Biotechnol Rep | Volume 1, Issue 2

November 16-17, 2017 Atlanta, Georgia, USA

Annual Congress on

Mycology and Fungal Infections

Characterizing protein interactions promoting heme regulation of the JMJC

domaincontaining protein Gis1 in yeast

Purna Chaitanya Konduri

University of Texas, USA

H

eme plays versatile and fascinating regulatory roles for fundamental biological processes. Heme serves as a

signaling molecule for oxygen levels in yeast as heme function is entwined with molecular oxygen levels.

Heme and oxygen regulate the expression of many genes in eukaryotes by modulating activity of regulatory

proteins. In yeast, Gis1 is a DNA-binding transcriptional regulator belonging to the JMJD2/KDM4 subfamily

of demethylases. It is highly homologous to the mammalian JmjC domain-containing protein JMJD2B, which

plays an important role in histone demethylation, oxygen regulation, and hormonal signaling. Notably, recent

experiments in our lab showed that heme regulates Gis1 transcriptional and demethylase activities. Biochemical

studies indicate that heme binds directly to Gis1 (JmjN+JmjC domain, ZnF) and JMJD2B proteins. This study

aims to dissect the molecular interactions promoting heme regulation of Gis1 activity by characterizing Gis1-

interacting proteins. Our Affinity Purification Mass Spectrometry (AP-MS) studies indicate that Gis1 interacts

with different sets of proteins under conditions of hypoxia, low heme, and high heme. Together, our results show

that Gis1 represents a novel class of transcriptional regulators, with multiple interacting partners playing a role

in mediating heme signaling.

Biography

Purna Chaitanya Konduri has completed her BE in Biotechnology from PES Institute of Technology, Bangalore, India in 2008 and MS in Molecular and Cell

Biology from UT Dallas in 2014. She is currently pursuing her PhD in Molecular and Cell Biology at UT Dallas in Dr. Li Zhang’s Lab. She is working on

understanding the mechanism underlying heme regulation of transcription factors in yeast. Specifically, she works on characterizing protein interactions involved

in heme regulation of JMJC-domain containing transcription factor Gis1. She has co-authored in a journal article accepted in Nucleic Acids Research and also

in a book chaper.

pxk121430@utdallas.edu