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Microbiol Biotechnol Rep | Volume 1, Issue 2

November 16-17, 2017 Atlanta, Georgia, USA

Annual Congress on

Mycology and Fungal Infections

Genomics, transcriptomics, proteomics, and biochemistry of white-rot basidiomycete

Trametes hirsuta 072

Andrey R Pavlov

1

, Tatiana V Tyazhelova

1

, Konstantin VMoiseenko

1

, Daria VVasina

1

, Olga VMosunova

1

, Tatiana V Fedorova

1

, Lilya

G Maloshenok

1

, Elena O Landesman

1

, Sergei A Bruskin

3

, Nadezhda V Psurtseva

4

, Alexei I Slesarev

2

, Sergei A Kozyavkin

2

and

Olga

V Koroleva

1

1

A N Bach Institute of Biochemistry Russian Academy of Sciences, Russia

2

Fidelity Systems, Inc., USA

3

N I Vavilov Institute of General Genetics Russian Academy of Sciences, Russia

4

Komarov Botanical Institute, Russia

W

ood-rotting fungi are organisms with the highest natural capacity to degrade lignocellulose substrates,

which is enabled by complex systems of extracellular enzymes, whose expression and secretion depend on

the nature of the environment. We sequenced and assembled the complete genome of the white rot saprotrophic

fungus

Trametes hirsuta 072

(Basidiomycota, Polyporales) providing a framework for studies of gene transcription,

translation, and secretion of essential fungal proteins. The genome sequence is assembled in 13 chromosomes

and a circular mitochondrion, and it is partially annotated. Our transcriptomic and proteomic studies are focused

primarily on ligninolytic oxidases and enzymes providing degradation of cellulose and hemicellulose. Our results

identified seven laccase genes containing exons and introns, including the respective promoter regions. We found

18 ligninolytic peroxidase genes encoding nine putative lignin peroxidases, seven putative short manganese

peroxidases, and two putative versatile peroxidases. The expression of the genes at various conditions of the

fungal growth was studied on the transcriptomic and proteomic levels. Only a few genes encoding ligninolytic,

cellulolytic, and hemicellulolitic enzymes were expressed in large quantities providing specific response of the

fungus to the particular conditions of growth.

Biography

Andrey R Pavlov is currently working as a Research Scientist in Fidelity Systems, Inc., Gaithersburg, Maryland, USA.

ppav2002@yahoo.com