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Microbiol Biotechnol Rep | Volume 1, Issue 2

November 16-17, 2017 Atlanta, Georgia, USA

Annual Congress on

Mycology and Fungal Infections

A comparative study on the growth of

Aspergillus

species on formulated culture media

from food crop wastes and sabouraud dextrose agar

Janet Uchechukwu Itelima

University of Jos, Nigeria

I

n developing countries where conventional media used for the isolation and growth of microorganisms are

very expensive and sometimes inaccessible to researchers, there is a growing interest regarding the utilization

of agro-waste materials and other organic wastes for the formulation media used for cultivating microorganisms.

A comparative study was carried out to test the suitability of formulated culture media from food crop waste

materials (yam, sweet potato and potato peels) and that of a conventional medium for cultivating

Aspergillus

species isolated from different foodstuffs. Three formulated media which included Yam Glucose Agar (YPGA),

Sweet Potato Peels Glucose Agar (SPPGA) and Potato Peels Glucose Agar (PPGA) were prepared and used in

comparison with Sabouraud Dextrose Agar (SDA) which is a conventional culture medium. One gram of each of

the foodstuffs was disinfected using 1% Sodium hypochlorite solution (1% NaOCl) for one minute, followed by

three successive rinses in sterile distilled water after which they were coarsely crushed and plated directly unto

sterilized Sabouraud Dextrose Agar (SDA). To inhibit bacteria growth, antibiotic drug (gentamicin) in solution,

was added to the media. The

Aspergillus

species isolated from foodstuffs which included

Aspergillus

niger

,

Aspergillus flavus Aspergillus

tamari

and

Aspergillus

fumigatus

were aseptically inoculated in duplicate into the

three different formulated culture media including SDA which served as a control. The cultures were incubated

at room temperature (25ºC) for five days. The diameter of the fungal isolates on both the control medium and

formulated media was measured in mutual perpendicular direction to ascertain the redial growth, starting from

the second day to the fifth day of incubation. The four species of

Aspergillus

species isolated from different food

stuffs grew profusely on the different formulated media with the exception of YPGA which yielded poor radial

growth of the fugal isolates. Although the percentage radial growth of each of the organism on SPPGA and PPGA

did not differ significantly (p˃0.05) from each other,

A.

niger

and

A. fumigatus

yielded maximum percentage

radial growth of (100%) each on SPPGA and PPGA, while

A. flavus

and

A. tamari

yielded (100%) each only

on SPPGA. Fugal growth on YPGA gave lowest percentage radial growth of 50.7, 50.2, 48.6 and 43.5% for

A.

niger

,

A. fumigatus

,

A. flavus

and

A. tamari

respectively. All the species of Apergillus yielded 100% radial growth

on the control (SDA). As the formulated media, especially (SPPGA and PPGA) compared favourably with the

conventional medium (SDA) in the terms of the radial growth exhibited by the different species of

Apergillus

, it

is therefore a clear indication that they could be good alternative culture media for the cultivation of these fungal

isolates.

Biography

Janet Uchechukwu Itelima has her expertise in Applied Microbiology and passion in research related to Applied Microbiology, Biotechnology, and Plant Science,

lecturing, and community services. She has obtained her PhD and she is currently an Associate Professor of Applied Microbiology. She is an Academic Staff

of the Department of Plant Science and Technology, Faculty of Natural Sciences University of Jos, Nigeria. She has published 35 papers both nationally and

internationally. She has also written two books. She is deeply involved in motivating students on how to obtain academic excellence. She has attended workshops

and conferences both nationally and internationally, where she presented papers, chaired sessions and served in advisory committee.

janetitelima@yahoo.com