microbiology-fungal-infections-2019-posters-abstracts

12 / 14

Page 34
Volume 3 Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2 nd Annual Congress on
6th International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
Chemical composition and antibacterial activity of Ageratina jahnii and Ageratina
pichinchensis essential oils collected in Mérida, Venezuela
Janne Rojas, Torres Lizzeth, Alexis Buitrago, Judith Velasco, Antonio Morales, Luis Rojas and María Lucena
University of Los Andes, Venezuela
Statement of the Problem: Ageratina genus belongs to Asteraceae family and is distributed in Colombia, Ecuador, Guatemala,
México, Panamá and Perú. In Venezuela are located at Amazonas, Aragua, Bolívar, Distrito Federal, Monagas, Zulia, Táchira,
Mérida and Trujillo, mainly between 1000 to 3850 m.a.s.l. Species of this genus have been used in traditional medicine for
the treatment of superficial mycosis, skin infections and wounds, as well as for its analgesic activity. Previous investigations
have revealed anti-inflammatory, antiviral, antibacterial and larvicidal activities either in extracts or isolated compounds from
different Ageratina species. Present investigation aims to compare the chemical composition and evaluate the antibacterial
activity of essential oils of A. jahnii and A. pichinchesis collected from Mérida-Venezuela.
Methodology & Theoretical Orientation : Fresh leaves of each species were cut into small pieces and submitted to
hydrodistillation for 4 h, using a Clevenger-type apparatus. The oils were dried over anhydrous sodium sulfate and stored at 4
ºC. Gas chromatography-Mass spectrometry (GC-MS) analyses were carried out on a Hewlett Packard GC-MS system, model
5973. The identification of components was based on a Wiley MS data library (6th ed), followed by comparisons of MS data
with published literature. The antimicrobial assay was carried out according to the disc diffusion method. MIC was defined as
the lowest concentration that inhibited the visible bacterial growth.
Findings: Essential oil from leaves of A. jahnii (AJ) and A. pichinchensis (AP) yielded 0.50% and 0.43 % w/v, respectively.
The major components identified in AJ were β-myrcene, α-pinene and limonene while for AP 8,9-epoxythymyl isobutyrate,
germacrene-D, thymyl isobutyrate and encecalol were observed as main compounds. Essential oils showed antibacterial activity
against S. aureus and E. faecalis with MIC values of 49.5 µl/mL for AJ and 104 µl/mL for AP.
Conclusion & Significance: Now days the study of antibacterial agents has become an important issue, due to the constant
development of resistance from microorganisms to conventional antimicrobials. Consequently, search for new agents, those of
plant origin must be emphasized, thus, results observed in this investigation might be of interest for the natural products research.