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Volume 3
Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2
nd
Annual Congress on
6
th
International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
Molecular and morphological identification of isolated fungal pathogens from infected
Nephelium lappaceum
L. (RAMBUTAN)
Alvarez Lourdes V, Tamada, Alyssa Marin C, Pagud, Rhenzie O
Polytechnic University of the Philippines, Philippines
N
ephelium lappaceum
L. (Rambutan) is a staple, seasonal tropical fruit that has both economical and medicinal value. It is
a fragile fruit that is sensitive to environmental and pathologic conditions. Considering the factors involved, the primary
reason for fruit rot is due to pathogenic fungi which cause infection and diseases. This study identified the fungi isolated from the
symptomatic leaves and fruits of rambutan. Infected plant samples were collected from a farm in San Juan, Sta. Cruz, Laguna.
Symptomatic tissues from leaves and fruits were cultured in potato dextrose agar and then transferred to malt extract agar to aid
sporulation. The fungal isolates, after subjecting for pathogenicity test, were characterized using the partial Internal Transcribed
Spacer (ITS) region and morphological analysis. DNA sequences of the isolates were subjected to phylogenetic analysis using
Mega v.7 software with reference sequences from the GenBank. The fungal pathogens were identified as
Aspergillus aculeatus,
Fusarium fujikuroi, Lasiodiplodia theobromae, Neopestalotiopsis clavispora, Pestalotiopsis microspora,
and
Trichoderma
afarasin
. The latter was found to be not pathogenic but exhibits biocontrol potential in plant host. Phylogenetic findings showed
that there is a high correlation of each isolated species to the published identified sequences in GenBank. The use of multilocus
analysis which may strengthen the species identification as well as testing the pathogenicity of the fungal isolates to rambutan
fruit is highly recommended.
lvalvarez@pup.edu.ph