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Volume 3
Microbiology 2019 & Fungal infections 2019
October 07-08, 2019
Journal of Clinical Microbiology and Infectious diseases
October 07-08, 2019 | Madrid, Spain
MICROBIOLOGY AND MICROBIOLOGISTS
MYCOLOGY AND FUNGAL INFECTIONS
2
nd
Annual Congress on
6
th
International Conference on
&
J Clin Microbiol Infect Dis, Volume 3
Study on stability of soil
Pseudomonas
and
Xanthomonas
plasmids possessing antibiotic
resistance genes
Nelli Hovhannisyan
1,3
, B G Babayan
1,2
, A S Sargsyan
1
, S A Bagdasaryan
1
and
A M Hovhannisyan
3
1
Scientific and Production Center “Armbiotechnology” NAS RA, Armenia
2
National Polytechnic University of Armenia, Armenia
3
Yerevan State University, Armenia
T
he emergence of multidrug-resistant (MDR)
P. aeruginosa
is a critical problem in medical practice, however the key
features involved in the emergence and spread of MDR
P. aeruginosa
remain unknown. Plasmids play the main role in rapid
dissemination of resistance and virulence genes by mechanism of genes horizontal transfer. The spread of antibiotic resistance
(ABR) has close relations with stability of plasmids possessing that features. To control the process of dissemination of MDR
strains it is important to understand how resistance genes remain stable in environment.
The stability of plasmid isolated from MDR soil
Pseudomonas
and
Xanthomonas
strains has been studied. All studied strains are
included in culture collection of MDC SPC “Armbiotechnology NAS RA. The MDR and the presence of
blaOXA-10
, aac(6’)II,
aph(3’)IV
in the genome of these strains were studied earlier.
Plasmids isolated from MDR strains were used for transformation of sensitive
P. aeruginosa
9056 strain and
E. coli
DH5α
strain. Antibiotic resistant colonies have been selected and passed over series of cultivation in antibiotic-free medium in order to
determine loss of plasmids (resistance). Results are presented in the Table.
Plasmids containing in
P. aeruginosa
9056 transformants have demonstrated high level of stability. However,
E. coli
transformants
have lost ABR after transfer to antibiotic medium. Thus, probably stability of
Pseudomonas
and
Xanthomonas
ABR plasmids is
under the host control.
Biography
Nelli Hovhannisyan has her expertise in biotechnology. The one of her scientific interests is combat against multidrug resistance
bacteria maintenance and spread in environment. She has experience in research, teaching and administration in education institutions
including Universality. As a head of Ecological safety lab, she manages the projects connected with ecology and biotechnology.
nelliog@yahoo.fr